Effect of Irradiation on Uricase Produced by Two Strains of Asperigillus niger
Usama F. Ali and Zeinab M. Ibrahim
Biological and Geological Dept. Faculty of Education, Ain Shams University, Cairo, Egypt
Abstract: The main purpose of this research is to screen the available local fungal strains for their uricase producing ability, to select the most potent isolate and also to optimize the culture conditions for maximum uricase production. Two strains of Aspergillus niger; A. niger (Thom) Thom & Raper 1945 which is thermotolerant and A. niger van Tieghem 1867 which is mesophilic were used during this study. Uric acid medium was the best for uricase production (2.14 and 2.45 U/ml); sucrose and starch were the best carbon sources (2.1 & 2.77 U / ml) while casein and peptone were the best nitrogen source (4.86 and 3.16 U/ml) ; alkaline medium (pH 9.0 & 8.0) were the best for the experimental fungi (4.42 and 5.27 U/ml); higher temperatures (45º & 35ºC) were the best the experimental fungi (4.65 and 5.11 U/ml ) . Low dose of Gamma radiation increased the production of uricase (0.5 & 0.3 KGy) gave the best results for uricase production (11.82 and 12.32 U/ml).
[Usama F. Ali and Zeinab M. Ibrahim. Effect of Irradiation on Uricase Produced by Two Strains ofAsperigillus niger. Life Sci J 2013;10(1):962-967]. (ISSN: 1097-8135). http://www.lifesciencesite.com.
Keywords: Aspergillus niger – Uricase production – Gamma irradiation.