Effects of Nigella sativa Oil and Thymoquinone on Renal Oxidative Stress and Apoptosis Rate in Streptozotocin-Diabetic Rats
Dalia A. Hafez
Nutrition and Food Science Department, Faculty of Design and Home Economics, Taif University, Kingdom of Saudi Arabia
Home Economics Department, Faculty of Education, Suez Canal University, Egypt
Abstract: The effects of Nigella sativa oil (NSO) and thymoquinone (TQ) on diabetes, renal oxidative stress, lipid peroxidation and apoptosis rate were investigated in streptozotocin (STZ)-diabetic rats. Forty five Sprague Dawley rats were randomly distributed into 5 groups (n=9): healthy control; diabetic control; diabetic given NSO (1ml/kg); diabetic given TQ (3 mg/kg) and diabetic co-administered with NSO and TQ, orally for 4 weeks. Diabetes was induced by a dose of STZ (50 mg/kg, i.p.) in fasted rats. Blood samples were collected for biochemical analysis. Kidneys were removed for determination of tissue antioxidant capacity, lipid peroxidation and apoptosis rate. The results showed that oral co-administration of NSO and TQ significantly increased serum insulin and decreased serum levels of glucose, glycosylated hemoglobin (HbA1c), urea, creatinine and alkaline phosphatase in diabetic treated rats. Treatment with NSO and TQ significantly increased activities of antioxidant enzymes (SOD, GPx and CAT), increased serum level of reduced glutathione (GSH) and decreased lipid peroxidation and apoptosis index. In conclusion, NSO and TQ exhibit good antidiabetic and antioxidant effects; improve renal function and reduce both malondialdehyde (MDA) and apoptosis rate in diabetic rats. Therefore, NSO and TQ may be beneficial for diabetes mellitus associated with nephropathy due to oxidative stress.
[Dalia A. Hafez. Effects of Nigella sativa Oil and Thymoquinone on Renal Oxidative Stress and Apoptosis Rate in Streptozotocin-Diabetic Rats. J Am Sci 2013;9(3):327-333]. (ISSN: 1545-1003).http://www.jofamericanscience.org. 46
Keywords: Nigella sativa oil; Thymoquinone, Apoptosis; Kidney function; Lipid; peroxidation; Antioxidant; Full Text 46