Heat Shock Protein-70 and -27 Expressions as Parameters of Early Diagnosis and Disease Progression in Hepatocellular Carcinoma
Amal Fawzy1, Hatem Attia2, Fatma A Khalaf3, Eman Abd El Sameea4, Mahmoud A El Tahawy4, Mohamed Farag4 and Fatma Younis5
Departments of Clinical Pathology1, National Cancer Institute-Cairo University
Clinical Pathology2, Biochemistry3 & Hepatology4, National Liver Institute-Menoufyia University & Tropical Medicine5, Faculty of Medicine- Al Azhar University
Abstract: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Despite remarkable advances in diagnostic and therapeutic techniques, the incidence of HCC is still on the increase. The role of liver biopsy in diagnosis of HCC has declined. However, with recent advances in genomics and proteomics a great number of potential serum and tissue markers have been identified and are being developed as new candidate markers for both diagnosis and prognosis of HCC, and may increase the need for liver biopsy. The aim of this study is to investigate the role of HSP70 and HSP27 expressions in early detection of HCC and to find their relation to parameters of disease progression. The study was conducted on 76 patients, 42 have proved HCC and 34 patients with liver cirrhosis (LC) without HCC. Routine laboratory investigations were done including: liver function tests, complete blood counts, serum alpha fetoprotein (AFP), hepatitis B and HCV hepatitis markers, and HCV-RNA levels. HSP70 and 27 expressions were determined in liver sampling by Real-Time PCR. Overexpression of HSP70 was detected in 92.86% of HCC which is statistically significantly higher compared to LC (2.94%) cases, and overexpression of HSP27 was significantly increased in HCC cases (57.14%) as compared to LC patients (8.82%). The overexpression of HSP70 was associated with early HCC diagnostic parameters (tumour size) and prognostic criteria (vascular invasion and tumour grade, Overexpression of HSP27 was associated with tumor size and tumor number, but not associated with each of AFP, vascular invasion and tumor grade. Conclusion: From the above results, we conclude that, found expressions of HSP70 and HSP27 may play an important role in hepatocarcinogenesis, and especially HSP70 can contribute tumor progression. We thus suggest that HSP70 may represent a good molecular target for treatment of HCV-related HCC.
[Amal Fawzy, Hatem Attia, Fatma A Khalaf, Eman Abd El Sameea, Mahmoud A El Tahawy, Mohamed Faragand Fatma Younis. Heat Shock Protein-70 and -27 Expressions as Parameters of Early Diagnosis and Disease Progression in Hepatocellular Carcinoma. Life Sci J 2013;10(1):262-268] (ISSN:1097-8135).http://www.lifesciencesite.com. 40
Key Words: Hepatocellular carcinoma, HSP70, HSP27, Expression, RT-PCR. Full Text 40