Eman Hamed1 and Aisha Elattar*2
1Department of Botany and Microbiology, Faculty of Science, Alexandria University
2 Department of Dairy Sciences and Technology Faculty of Agriculture, Alexandria University. Egypt.
Abstract: A study was carried out to investigate the probiotic potential of isolated lactic acid bacteria from camel’s row milk collected from Arabian camels (Camelus dromedaries) in Egypt. Eleven gram positive, catalase negative isolates were identified using API 20STREP identification system for the identification of cocci isolates and API 50CHL for bacilli. Isolates were identified as Enterococcus faecium (seven isolates),Enterococcus durans (one isolate), Aerococcus viridians (one isolate), Lactococcus lactis (one isolate) andLactobacillus plantarum (one isolate). The probiotic potential of these isolates was investigated using in vitroantagonistic tests against Salmonella typhi ATCC 14028, E.coli ATCC 25922 and Vibrio fluvialis using agar spot test. All of the isolates were proved to be effective against those pathogens. Isolate ES08 was able to inhibit the growth of indicator pathogens with an average inhibition zone of 3.3, 3.7 and 2.0 cm in diameter against Salmonella typhi, E.coli and Vibrio fluvialis, respectively. All of the isolates showed resistance to stomach pH (pH 3.0), tolerance against 0.3% bile salts concentration and none of the isolates caused blood hemolysis. Isolate ES08 was farther identified by sequencing their 16S rRNA encoding gene.
[Eman Hamed and Aisha Elattar. Identification and Some Probiotic Potential of Lactic Acid Bacteria Isolated From Egyptian Camels Milk. Life Sci J 2013;10(1):1952-1961] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key Words: Camels milk, Lactic Acid Bacteria, Probiotic potential.