Proteomic Profiling and Protein Identification by 2-DE gel electrophoresis combined with MALDI-TOF Mass Spectrometry in Rat hepatocyte Nucleus
Xianguang Yang1, 2, Chunling Yan1, 2, Dongdong Sun1, 2, Xiaofang Geng1, 2, Yanjie Yang2, Cunshuan Xu1,2*
1. College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China
2. Co-constructing Key Laboratory by Province and the MOST for Cell Differentiation Regulation, Xinxiang, Henan 453007, China
Abstract: More and more proteomics research focusing on nucleoprotein. In this article, the proteomic profiling of Rat hepatocyte nucleus was showed by 2-D PAGE and a total of 204 kinds of nucleoproteins were identified by MALDI-TOF Mass Spectrometry. Addition to housekeeping protein, low kurtosis transcription factors were enriched and identified too. Ingenuity Pathway Analysis (IPA) showed that these nucleoproteins were involved in the rat liver cell cycle regulation, DNA replication, recombination and damage repair, cell assembly and organization, cell development, nervous system development and function, cancer, cardiovascular system development and function, cell death and survival, cell morphology, Cell-To-Cell signaling and interaction. These results can provide more information for a comprehensive understanding to the function of nucleus; on the other hand, the results lay the foundation for the analysis of nucleoproteins expression varieties and help to look for the biomarker of disease diagnosis and therapies.
[Xianguang Yang, Chunling Yan, Dongdong Sun, Xiaofang Geng, Yanjie Yang, Cunshuan Xu. Proteomic Profiling and Protein Identification by 2-DE gel electrophoresis combined with MALDI-TOF Mass Spectrometry in Rat hepatocyte Nucleus. Life Sci J 2013:10(1):3183-2191]. (ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: Rat hepatocyte, Nucleoprotein, Proteomics, 2-D gel electrophoresis, MALDI-TOF MS.