The expression of UDP-glucose pyrophosphorylase 2 following acute myocardial infarction in rat
LuShun Zhang 1, LinBo Gao 2, WeiBo Liang 1, YunLiu 1, TianYi Chen 1 and Lin Zhang 1
1 Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China.
2 Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China
Abstract: Acute myocardial infarction (AMI) is a major cause of sudden cardiac death (SCD) in patients with coronary and ischemic heart diseases. However, the age of AMI estimation in SCD is not fully known. The aim of this study was to investigate the expression of UDP-glucose pyrophosphorylase 2 (UGP2) in AMI. A total of 71 Sprague–Dawley male rats were divided into AMI group, sham-operated group and normal control group. After surgery, the myocardium was harvest following 0 min, 15 min, 30 min, 60 min, 120 min, 240 min in AMI group and sham-operated group. The expression of UGP2 in myocardium of rats was detected using quantitative real-time PCR and Western Blot. UGP2 mRNA and corresponding protein expression were decreased after ligation 15 min, and significant differences were found in UGP2 mRNA (after ligation from 30 min to 120 min) and UGP2 protein (after ligation from 30 min to 240 min) in the AMI zone compared with the control, respectively. These findings suggest that UGP2 may be used as a potential biomarker for the time estimation in AMI.
[LuShun Zhang, LinBo Gao, WeiBo Liang, YunLiu, TianYi Chen, Lin Zhang. The expression of UDP-glucose pyrophosphorylase 2 following acute myocardial infarction in rat. Life Sci J 2013;10(1):3299-3302] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key words: UGP2; AMI; Age of AMI; SCD.