Screening of Some Antibiotics and Anabolic Steroids Residues in Broiler Fillet Marketed in El-Sharkia Governorate
Mohamed Abdallah Hussein1 and Samah Khalil 2
1Food Control Dept., 2Forensic Medicine and Toxicology Dept., Faculty of Veterinary Medicine, Zagazig University, Egypt. Samah_vet2001@yahoo.com
Abstract: Antibiotics and anabolic steroid growth promoters usage have facilitated the efficient production of poultry, allowing the consumer to purchase, at a reasonable cost, high quality meat and eggs. Although these uses beneﬁt all involved, unfortunately, the edible poultry tissues had harmful concentrations of drug residues. Therefore, this study was carried out on one hundred randomly collected fresh and frozen broiler fillet samples (50 of each) to evaluate the antibiotic residues level qualitatively by microbiological inhibition assay followed by quantitative detection for oxytetracycline and enrofloxacin by high performance liquid chromatography (HPLC). In addition to monitoring of anabolic steroids (Testosterone, Progesterone and Zeranol) quantitively by Enzyme- linked immunosorbent assay (ELISA). The obtained results revealed a detectable level of oxytetracycline residues which confirm widespread misuses of antibiotic especially oxytetracycline in farms and lack of application of recommended withdrawal times. The anabolic steroids residues level including testosterone and progesterone were within the permissible limit which refers to no illegal use of hormones as growth promoting agents in broiler production. None of the samples displayed the presence of zeranol residue as the level of it was below the detection limit of the used kits (<100 ng/kg). For monitoring the effect of cooking process on antibiotic residue levels in broiler fillet, ten broilers (40 days old) were classified into two groups, each group was dosed over 5 consecutive days with 15 mg/ kg day of oxytetracycline or enrofloxacin. Five slices of broiler fillet from each group subjected to cooking either via frying or grilling.The results showed that cooking had an effect in reducing the concentration of antibiotic residues as there are a signficant reduction percentages of oxytetracycline while enrofloxacin residues showed low reduction percentages. These findings recommended that restricted measures and harder regulations must be applied to prevent the misuse of drugs in poultry industry, application of withdrawal time as well as the inspection of chickens for drug residues prior to marketing.
[Mohamed Abdallah Hussein and Samah Khalil. Screening of Some Antibiotics and Anabolic Steroids Residues in Broiler Fillet Marketed in El-Sharkia Governorate. Life Sci J 2013;10(1):2111-2118] (ISSN:1097-8135). http://www.lifesciencesite.com. 9
Key words: Antibiotic, Anabolic steroid, Residue, Maximum residue limit, Microbiological inhibition test, HPLC, ELISA.