Comparative Molecular Analysis of Egyptian Strains of Highly Pathogenic Avian Influenza (HPAI) Virus H5N1 Isolated During 2006 And 2007
Madbouly, H.M1; Orkhan, M.H2; Nagwa El-Kholy2 and Hussein, A.S1.
1Department of Virology Faculty of Veterinary Medicine, Beni-Suef University, Egypt
2World Health Organization National Influenza Center (WHONIC), VACSERA, Egypt
Abstract:Isolated strains of HPAI H5N1 during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Egypt were molecularly characterized and compared with those isolated strains during January 2007 from the same locality. The isolated strains from infected chicken during 2006 were closely related to strain isolated from turkeys located in Turkey at 2005 (A/ty/Turkey/1/05) and toA/Vietnam/1194/04 strain isolated from human cases of AI H5N1 and induced higher case fatality in human in Vietnam. The phylogenetic analysis of HA and NA genes of the isolated strains during 2006 revealed that, these isolates are located in Eurasia- African lineage which contains AI H5N1 strains from Egypt, Turkey, Romania, Iraq, Mongolia, Iran, Korea and Nigeria and differed from those isolated in South-Eastern of Asia which contains Cambodia, Thailand, and Vietnam Nam strains of AI H5N1. The phylogenetic analysis of HA, NA, and M genes of Egyptian isolates of January 2007 from the same locality showed minor antigenic variation from those of 2006 and located in a new sub-clade from Egyptian strains of 2006 and all of them located in Euro-African lineage. All 8 genes were closely related to the genes of onther Egyptian H5N1 isolates from chicken, duck, and human. The sequence of NAs did not possess any know oseltamivir resistance mutations. Also the M2 sequences did not possess amantadine resistance mutations.
[Madbouly, H.M; Orkhan, M.H; Nagwa El-Kholy and Hussein, A.S. Comparative Molecular Analysis of Egyptian Strains of Highly Pathogenic Avian Influenza (HPAI) Virus H5N1 Isolated During 2006 And 2007. J Am Sci 2012;8(10):254-240]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 38
Key words: Egyptian strains - highly pathogenic- avian influenza (HPAI) virus H5N1 Full Text 38