Effect of Combination of L-Arginine and N-Acetylcysteine in Rat Model of Renal Ischemia-Reperfusion Injury
Abeer A.Abo Zeid1, Mervat H. El Saka1, and Noha M Shafik2
Physiology Department1, Biochemistry Department 2Faculty of Medicine, Tanta University - Egypt
Abstract:. Background: Ischemia, followed by reperfusion (I/R) is one of the major causes of acute renal failure (ARF). The pathogenesis of I/R is multifactorial .Nitric Oxide(NO) proved to be closely related to the pathogenesis of I/R-induced ARF .Therefore, the aim of the present study was to analyze the role of NO-dependent mechanisms in the renal response to I/R by providing a source of(NO) precursor (L-Arginine) in the presence or absence of N-acetylcysteine (NAC)which protect NO from interacting with oxygen free radicals that may contribute to the pathogenesis of ischemia reperfusion injury. Materials and Methods: Forty male Sprague-Dawley rats were submitted to right nepherectomy and divided into four groups: control, renal I/R, renal I/R withL-Arginine, renal I/R withL-Arginine and NAC, I/R injury was induced by 45 min of left renal artery ischemia followed by60 min of reperfusion. After reperfusion, 24 hours urine was collected to measure creatinine and para amino hippuric (PAH) clearance and urinary nitrites. Blood samples were collected to measure blood urea nitrogen (BUN) and serum creatinine. All animals are scarified, the left kidney are removed to measure protein content, superoxide dismutase, malondialdehyde and glutathione in the renal tissue, histopathological examination for the left kidneyswere performed. Results: Renal I/R resulted in significant elevation in blood urea, serum creatinine and renal tissue malondialdehyde and significant lowering in superoxide dismutase, kidney glutathione, PAH clearance, creatinine clearance and urinary nitrites secretion. L-Arginine alone result in significant improvement in renal function parameters when compared to I/R group but this improvement is still insignificant when compared to control group also parameters of oxidative stress and urinary nitrites secretion does not significantly affected., but in the renal I/R+ L-Arginine and NAC, all biochemical results and histopathological parameters were significantly improved as compared to control group.Conclusions: Combined treatment with antioxidant (NAC) and nitric oxide precursors (L-Arginine)attenuates renal ischemia-reperfusion injury, by direct scavenging of reactive oxygen species (ROS), thus protecting NO from reaction with it.
[Abeer A.Abo Zeid, Mervat H. El Saka, and Noha M Shafik. Effect of Combination of L-Arginine and N-Acetylcysteine in Rat Model of Renal Ischemia-Reperfusion Injury. J Am Sci 2012;8(10):814-821]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 110
Key words: Ischemia reperfusion (I/R), L-Arginine, Nitric Oxide (NO), N-acetylcysteine and experimentalrats Full Text 110