Genetic Diversity and Phylogenetic Relationship among Some Rabbit Breeds Using Random AmplifiedPolymorphic DNA Markers
El-Bayomi Kh. M.1, Awad A.1* and Saleh A. A.1
1 Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University
Abstract: Random Amplified Polymorphic DNA (RAPD) marker was employed to assess the genetic variation and phylogenetic relationship among three rabbit breeds viz. New Zealand White, Californian and Flanderreared in Egypt. Initially, a total of 20 random primers of arbitrary sequence were used but 14 of them generated reproducible, scoreable and polymorphic bands. Out of 120 bands scored using these primers, 39 (33%) were recognized as polymorphic and 81 (67%) as monomorphic bands. The highest percentage of polymorphic bands was recognized for primers OPA-10 and OPA-06 (56%) while the lowest percentage of polymorphic bands was recognized for primers OPE-19 (7%) and OPF-12 (14%). The band sharing frequencies (BSF) was found higher between New Zealand White-Californian (0.88±0.029), followed by Californian-Flander(0.87±0.024) and New Zealand White-Flander (0.84±0.034). Overall, there was no significant difference (P>0.05) in BSF values between breeds. The highest genetic distance was found between New Zealand White-Flander(5.568) followed by Californian-Flander (5.000) and New Zealand White-Californian (4.690). One primer (OPE-11) in New Zealand White, three primers (OPA-10, OPC-02, OPF-09) in Californian, five primers (OPA-01, OPA-06, OPA-10, OPB-14, OPF-09) in Flander were found to be specific for these breeds. The study suggests that RAPD can be successfully utilized for detecting genetic variation among the studied rabbit breeds.
[El-Bayomi Kh. M., Awad A. and Saleh A. A. Genetic Diversity and Phylogenetic Relationship among Some Rabbit Breeds Using Random Amplified Polymorphic DNA Markers. Life Sci J 2013;10(1):1449-1457]. (ISSN: 1097-8135). http://www.lifesciencesite.com.
Key words: Rabbit, breed, RAPD markers, genetic diversity, phylogenetic.