In Vivo Stu
In Vivo Study of the Effectiveness of Ozonized Olive Oil Gel on Inhibiting Enamel Demineralization during Orthodontic Treatment
Safaa Ali Ghobashy1 and Heba Mohamed El-Tokhey2
1Department of Orthodontics, Faculty of Dentistry, Tanta University; 2 Department Oral biology, Faculty of Dentistry, Tanta University. email@example.com
Abstract: The aim of this study was to evaluate the effects of ozonized olive oil gel in reducing enameldemineralization around orthodontic bracket during orthodontic treatment, and to compare it with the control. Methods: Fourteen patients scheduled to have premolars extraction as a part of orthodontic treatment were participated in the present study, aged 15- 18 years. The patients were randomly divided into 3 groups; control group (16 upper and lower premolars) and two experimental groups (20 upper and lower premolars for each). In Control group no treatment was applied on the premolars. In experimental groups (I and II), after etching and bracket bonding, T-loops were formed with 0.014 inch stainless steel wire and engaged on the experimental teeth. In experimental group II, ozonized olive oil gel was applied sparingly to the tooth surface with concentration of 20:25 µgm/ml. Participants were instructed to apply the ozonized olive oil gel to the buccal surface of the premolars three times daily for 8 weeks after regular oral hygiene procedures. After 8 weeks, the brackets were deboned and the premolars extracted carefully. The premolars of each group were divided into 2 equal numbers; 28 premolars prepared for elemental analyses (calcium and phosphorus) followed by scanning electron microscope investigation, and 28 premolars examined with polarized light. ANOVA test was applied followed by LSD post hoc test to compare between each 2 groups. Results: ANOVA test showed statistical significant differences for Ca/P ratio between all groups (p <0.001). Polarized light and scanning electron microscopic examinationrevealed that ozonized olive oil gel was efficient in reducing enamel demineralization with almost normal appearance of perikymata and new hydroxyapatite crystal deposition. Conclusion: The use of ozonized olive oil gel in addition to the standard oral hygiene regimen was found to be beneficial for orthodontic patients to prevent enamel decalcification during treatment.
[Safaa Ali Ghobashy and Heba Mohamed El-Tokhey. In Vivo Study of the Effectiveness of Ozonized Olive Oil Gel on Inhibiting Enamel Demineralization during Orthodontic Treatment. J Am Sci2012;8(10):657-666]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 89
Keywords: Demineralization, ozone, polarized light, scanning electron microscope.