Association between CC16 Polymorphism and Bronchial Asthma
Nisreen M.El Abiad1, Hisham Waheed2, William M. Morcos2, Samar M. Salem2, and Hala Ataa3, Olfat G. Shaker4
Departments of: 1Pediatric, 4Medical Biochemistry, Faculty of Medicine, Cairo University, 2Child Health, NRC. And 3Al Galaa Teaching Hospital, Cairo, Egypt. email@example.com
Abstract: Background: Asthma is one of the most common chronic pediatric diseases, and is responsible for a significant proportion of school day losses. Asthma is influenced by genetic and environmental factors. The inheritance pattern of asthma demonstrates that it is a complex genetic disorder. Clara cell secretory protein (CC16) is an ideal candiadate for involvement in an inherited predisposition to asthma owing to its chromosomal location, nature and function. Objective: to screen exons of CC16 gene on chromosome 11 for detection of sequence variation in families to determine whether these allelic polymorphism are associated with clinical asthma or not and to detect the relation between type of genetic polymorphism and asthma severity. Patients and Methods: This study included 20 stable asthmatic children with 21 of their atopic family members, also 11 healthy non atopic subjects were included as control group, all cases were subjected to genetic study methods including pedegree construction, PCR and detection of allelic polymorphism. Results: Significant correlation (90%) between homozygous CC16, 38 (AA) (75%) and 38 (AG) (15%) allelic polymorphism and increased prevalence of asthma was detected in families but there was no correlation between CC16 allelic polymorphism and asthma severity. Conclusion: There was a correlation between CC16 allelic polymorphism and increased prevalence of asthma and this gene is present also in normal subjects who are not triggered by environmental factors.
[Nisreen M.El Abiad, Hisham Waheed, William M. Morcos, Samar M. Salem, and Hala Ataa, Olfat G. Shaker. Association between CC16 Polymorphism and Bronchial Asthma. Life Sci J 2012;9(1):265-270]. (ISSN: 1097-8135).http://www.lifesciencesite.com. 36
Key words: CC16- Gene polymorphism – PCR- Asthma Full Text 36