Effect of Vi
Effect of Vitamin E on Sperm Parameters, Chromatin Quality and Testosterone Hormone in Mice
Pourentezari M 1 M.Sc, Talebi AR1, 2 Ph.D, Anvari M*1, 2 Ph.D, Khalili MA1, 2 ph.D, Mangoli E1 M.Sc,
Address: Department of Biology & Anatomy1, Research & Clinical Center for Infertility2, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
*Corresponding author: firstname.lastname@example.org
Abstract: The present study is carried out to investigate the protective effect of vitamin E (vit.E) onchromatin quality and sperm parameters (count, motility, viability and morphology) in mice. Antioxidants are the main defense factors against oxidative stress induced by free radicals. Vitamin E is believed to be the primary component of the antioxidant system of the spermatozoa and is one of the major membrane protectants against ROS and LPO attack. There is a relationship between activity of these antioxidant and function of sperm. Vitamins E and C which are belong to non-enzymatic antioxidant are used as a supplemented drug to improve sperm quality in male infertility. Material and methods: 16 adult male mice were divided equally into two groups each containing 8 mice .mice of group 1 served as control fed on basal diet, group 2 received basal diet and vitamin E (100 mg/kg, intraperitoneal) for 35 days. Blood was taken for the determination of serum testosterone. Finally, right tail of epididymis was cut in Ham’s F10. Released sperm were used to analyze number, motility, morphology (Pap-staining) and viability (Eosin-Y staining) of the sperm and DNA integrity and chromatin condensation assessments were ready by standard cytochemical techniques including. (AOT): Acridine orange is a metachromatic fluorescence explore for the dimension of sperm nuclear DNA susceptibility to in situ acid-induced denaturation by distinctive between double-stranded DNA and single-stranded DNA. (AB): Aniline blue selectively stains lysine-rich histones and has been used for the purpose of those sperm chromatin condensation anomalies that are related to residual histones. (TB): Toluidine blue is a metachromatic dye which determines both the quality and the quantity of sperm nuclear chromatin. Result: In vitamin E mice, a significant increase was found in sperm number, sperm motility, sperm viability and sperm morphology compared to control group. A significant increase was also found in sperm AB+ (Mature sperm), TB- (normal) and Ao+ (double-stranded DNA) in vitamin E group compared to control group .the results suggest an increase in concentration testosterone in vitamin E mice compared to control mice,Conclusion: It was concluded, Vit.E not only is able to improvement the sperm parameters but also increases sperm chromatin quality in mice. Also vitamin E increases the level of testosterone hormone in mice.
[Pourentezari M M.Sc, Talebi, AR Anvari M, Khalili MA, Mangoli E, M.Sc. Effect of Vitamin E on Sperm Parameters, Chromatin Quality and Testosterone Hormone in Mice J Am Sci 2012;8(10):15-21]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 4
Key words: Mice, Vitamin E, DNA integrity, Sperm parameters, Testosterone. Full Text 4