Histopathological effects of experimental phenylketonuria on 15 days albino rat placenta
Hala. M. Ebaid
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract: Phenylketonuria (PKU) is a genetic disorder that is characterized by an inability of the body to utilize the essential amino acid, phenylalanine. The disease results from a deficiency in phenylalanine hydroxylase, the enzyme catalyzing the conversion of phenylalanine to tyrosine. Although this inborn error of metabolism was among the first in humans to be understood biochemically and genetically, little is known of the mechanisms involved in the pathology of PKU during neonatal development. Hyperphenylalaninaemia (Elevated concentrations of plasma phenylalanine) were induced in pregnant rats by oral administration of 30 mg. DL–α-methylphenylalanine/100 g (to inhibit maternal liver phenylalanine hydroxylase) plus phenylalanine supplementation at a dosage of 60 mg/100 g body weight two times daily (to increase maternal and fetal plasma phenylalanine) after 6th day of onset of gestation till 15 days of gestation. Treatment with alpha-methylphenylalanine/phenylalanine affect placentation through reduction in placental weight and histopathologically, through increase in apoptotic cells in the labyrinth zone and basal zone, and hypoplasia of the labyrinth zone and basal zone, dilatation of the blood vessels and inducing haemorrhagic and degenerative changes in the layers of placenta. Conclusions: PKU affects placentation that may be reflected on the growth and development of fetuses.
[Hala, M. Ebaid. Histopathological effects of experimental phenylketonuria on 15 days albino rat placenta. J Am Sci 2013;9(6):379-386]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 45
Keywords: PKU, Hyperphenylalaninaemia, rat placentation. Full Text 45