Serum Fetuin-A in Egyptian Pediatrics with Chronic End-stage Renal Diseases: A Correlation to Vascular Inflammatory Biomarkers
*Mona F. Schaalan, * Lamiaa N. Hammad,*Sahar A. Raouf and **Maha A. Abo-Shadi and *** Waleed A. Mohamed
* Biochemistry Department, Faculty of Pharmacy, Misr International University **Microbiology and Immunology Dept., Faculty of Pharmacy, Al-Azhar University,***Chemistry Department, Kasr ELeini Teaching Hospitals, Cairo University, Cairo, Egypt. email@example.com
Abstract: Background: The incidence of vascular calcification is known to be elevated in haemodialysis patients. A close relationship does also exist between inflammation and vascular calcification. Aims: To understand role of inflammation; we investigated the serum concentrations of some inflammatory markers in children with end stage renal diseases, and compared those patients either undergoing haemodialysis or not with their control counterparts. The potential relationship between serum fetuin-A and the inflammatory markers in the studied patients was also unraveled. Methodology: In this prospective non-randomized study, eighty chronic kidney failure pediatric Egyptian patients were enrolled. Group I included 40 of them who were managed without haemodialysis, while group II included 40 on regular haemodialysis in the Center of Pediatric Nephrology and Transplantation (CPNT), Cairo University Children’s Hospital, Egypt; both groups were compared with a control group of 40 subjects with normal renal function. Serum fetuin-A, IL-1b, IL-23, TNF-α, circulating soluble intercellular (sICAM-1) and vascular cellular (sVCAM-1) adhesion molecules were measured by ELISA. Results: Comparing the hemodialized patients in group II with the non-hemodialized in group I, IL-1b was significantly lower in group II (p=0.023), whereas, other parameters showed insignificant decrease (sICAM-1, p=0.223, sVCAM-1, p = 0.209, TNF-α, p =0.059, IL-23, p =0.339, Fetuin-A, p =0.236). All inflammatory markers were significantly higher in both patient groups I and II, compared to their control, except for fetuin-A which showed a significant decrease. Statistically significant negative linear correlations were found between fetuin-A and all inflammatory markers among all studied patient groups and within each group separately.Conclusion: Our data suggest that the immune cells in hemodialized children are in an activated state which explains the induced alterations in the assessed immunological markers. An important negative associations between serum fetuin-A and serum inflammatory markers concentrations were also demonstrated.
[Mona F. Schaalan, Lamiaa N. Hammad, Sahar A. Raouf , Maha A. Abo-Shadi and Waleed A. Mohamed.Serum Fetuin-A in Egyptian Pediatrics with Chronic End-stage Renal Diseases: A Correlation to Vascular Inflammatory Biomarkers. Life Sci J 2013;10(1):1149-1156] (ISSN: 1097-8135). http://www.lifesciencesite.com.
Keywords: Chronic End-stage Renal Disease (ESRD), Pediatrics, Haemodialysis, Fetuin-A, adhesion molecules, Interleukins.