The Characterization of SmedHSP90 Gene Using Methods of Bioinformatics
Xingzi Xi1, Keshi Ma2
1. Department of Education Sciences, Xinxiang University, Xinxiang 453003, China,
2. College of Life Sciences, Zhoukou Normal University, Zhoukou 466000, China
Abstract: Bioinformatics is a very powerful tool in the field of genomics study. Firstly, we obtained the heat shock protein 90 gene structure of planarian Schmitdea mediterranea (designated SmedHSP90) by using Smedgenome database. The ORF of SmedHSP90 is 2148 bp encoding a polypeptide of 715 amino acids with allfive conserved motifs of HSP90 protein family signature. In addition, the gene structure of SmedHSP90contains a 48 bp intron, and the 5’- and 3’- splicing site follows the typical “GT-AG” rule. Secondly, we constructed the HSP90 phylogenetic tree using the software Clustal 1.83 and Mega 3.1, and discussed the evolutionary position of SmedHSP90. Thirdly, three-dimensional domain structure of SmedHSP90 was predicted by SWISS-MODEL Server. Bioinformatic analysis of SmedHSP90 gene provides basic data for the study of stress response in planarians.
[Xingzi Xi, Keshi Ma. The Characterization of SmedHSP90 Gene Using Methods of Bioinformatics. Life Sci J2013;10(1):2836-2839] (ISSN:1097-8135). http://www.lifesciencesite.com. 1
Keywords: bioinformatics, planarian, HSP90.