Detection of C
Detection of Circulating Microparticles in Patients with Proliferative Diabetic Retinopathy
Samy A Khodeir 1, Y M Abd El Raouf 1, Gihan Farouk2 and Mohammed EL-Bradey3
Departments of 1Internal Medicine 2Clinical Pathology and 3Ophthalmology, Faculty of Medicine, Tanta University.firstname.lastname@example.org
Abstract: Background: The development of vasculopathies in diabetes involves multifactorial processes including pathological activation of vascular cells. Release of microparticles by activated cells has been reported in diseases associated with thrombotic risk, but few data are available in diabetes. Diabetic retinopathy is associated with increased local activation or apoptosis of retinal, neural, and vascular `endothelial cells in the eye which indicate that microparticles (MPs) of different cellular origin might be locally generated in the eye of diabetic patients. Aim: The aim of this study is to investigate the presence of endothelial, platelet, and retinal-derived microparticles both in the vitreous and in the plasma of diabetic patients compared with that of non diabetic ones. Subjects and methods: In a case-control study, this study included 45 patients: 25 diabetic patients with non proliferative diabetic retinopathy (NPDR), and 20 diabetic patients with proliferative diabetic retinopathy (PDR) compared with control group consists of non diabetic 10 subjects. Blood samples were analyzed by flow cytometry. Microparticles present in plasma and vitreous were analyzed according to their parameters of size and fluorescence. Results: As regard plasma samples, there was significant increase in CD144 and CD41 in groups II, III as compared with control group (p = 0.001). Peanut agglutinin PNA was not detected in plasma sample among all studied groups. Whereas, as regard to the vitreous sample, there was significant increase in CD144, CD41 and PNA in groups II,III as compared with control group (p= 0.048, 0.009, 0.048 ), (P = 0.001) respectively. Conclusion: microparticles appear as a new prognostic potential of type 2 diabetes in the early detection of vascular complications. Moreover significant increase of different types of microparticles in vitreous fluid of membrane in patients with PDR, may contribute to disease progression.
[Samy A Khodeir , Y M Abd El Raouf , Gihan Farouk and Mohammed EL-Bradey. Detection of Circulating Microparticles in Patients with Proliferative Diabetic Retinopathy. Life Sci J 2012;9(1):204-209] (ISSN: 1097-8135).http://www.lifesciencesite.com. 29
Key words: Microparticles, diabetic retinopathy, endothelial cell dysfunction. Full Text 29