Hepato-protective Effect of Green Tea Extract on Cyclosporine a Treated Rabbits: Histological and Ultrastructural Study
Fathy Ahmed Fetouh and Amal Al Shahat
Department of Anatomy, Faculty of Medicine, Zagazig University
Abstract: Cyclosporine A (CsA) has a biologic activities, including anti-parasitic, fungicidal and anti-inflammatory effects by inhibiting T-cell activation. CsA and its metabolites are taken and excreted by the hepatocytes into the bile and a small portion into the blood. Green tea contains several antioxidants and has a role as an antioxidant. Objective: The present work aimed to study the structural hepato-protective effect of green tea extract on rabbits treated with cyclosporine A (CsA). Materials and methods: Three groups of adult rabbits were used for this study; the 1st group was used as a control, the 2nd group was treated with cyclosporine A (CsA) as oral solution in a dose of 15 mg/kg for 4 weeks and the 3rd group was treated with cyclosporine A in the same dose simultaneously with green tea extract for the same period. The animals were anaesthetized and liver specimens were obtained and semi-thin sections of 1 mm thick were obtained, stained with 1% toluidine blue and examined by light microscopy and ultrathin sections for electron microscopic examination. Results: The cyclosporine A had adverse effects on the liver cells of cyclosporine A-treated animals in comparison with that of the control group where, the hepatocytes showed extensive vacuolization and variable areas of fatty degeneration. Also, the cytoplasm showed reduced organelles, as few mitochondria with loss of their transverse cristae, few glycogen particles and loss of endoplasmic reticulum. In some cells the cytoplasm showed loss of the organelles and depletion of glycogen content. The nucleus appeared irregular with deformed nuclear membrane. A concomitant administration of green tea extract to cyclosporine A treated rabbits markedly prevented the adverse effects of cyclosporine A where the hepatocytes preserved their normal appearance. The cytoplasm showed numerous mitochondria with well developed transverse cristae, smooth and rough endoplasmic reticulum, Golgi apparatus and abundant amount of glycogen particles. The nucleus appeared round and light with well formed nuclear membrane. Also, bile canaliculi and the microvilli projecting from the cells were preserved. Conclusion: The green tea extract had beneficial effects against cyclosporine A induced hepatic toxicity and should be used in combination with cyclosporine A transplant treatment to improve the cyclosporine A induced oxidative stress parameters and other adverse effects.
[Fathy Ahmed Fetouh and Amal Al Shahat. Hepato-protective Effect of Green Tea Extract on Cyclosporine a Treated Rabbits: Histological and Ultrastructural Study. Life Sci J 2013;10(1):1924-1932] (ISSN:1097-8135).http://www.lifesciencesite.com.
Key words: Green tea extract, Cyclosporine A, Hepatocytes, Ultrastructure.