The Expression and Regulation of GSK-3β, CDK-5 and PP2A in Differentiated Neural Stem Cells of Rats
ZHAO Qing-xia1,3, XU Yan2,3, YAN Wen-hai2,3, HAN Xue-fei3, XING Ying2,3
1. The Nursing College; 2. The Essential Medical School; 3. The Stem Cells Research Center
Zhengzhou University, Zhengzhou, Henan 450052, China
【Abstract】 Objective: To explore the expression of GSK-3β, CDK-5 and PP2A and the regulation of them by Aβ25~35and ginsenoside Rb1 after NSCs are transformed into neurons. Methods Neural stem cells of the third passage were induced towards neurons; the expressions of GSK-3β (pTyr279, 216), PP2A and the regulation of them by Aβ25~35 and ginsenoside Rb1 were tested by the immunofluorescence cytochemical staining after NSCs had been induced for one week; The expressions of GSK-3β, CDK-5, PP2A and the regulation of them by Aβ25~35 and ginsenoside Rb1 were detected with RT-PCR. Results: Immunofluorescence cytochemisty showed that neural cells from NSCs which had been differentiated after one week can express GSK-3β (pTyr279, 216) and PP2A. Aβ25~35 can enhance the expression of GSK-3β (pTyr279, 216), meanwhile it also restrained the expression of PP2A. Moreover ginsenoside Rb1 can lessen the affect of Aβ25~35 . RT-PCR found that neural stem cells which had been differentiated after one week can express GSK-3β, CDK-5, PP2A. The expression of GSK-3β and CDK-5 rose up and the expression of PP2A weakened when they were treated by Aβ25~35. However, the effect of Aβ25~35 was restrained when they were pretreated by ginsenoside Rb1.Conclusions NSCs which were cultured and induced in vitro can express GSK-3β, CDK-5 and PP2A; moreover Aβ25~35and ginsenoside Rb1 can regulate the expressions of GSK-3β, CDK-5 and PP2A. It hinted that cells which differentiated from neural stem cells in vitro have protein phosphorylation regulation system of normal cells.
[ZHAO Qing-xia, XU Yan, YAN Wen-hai, HAN Xue-fei, XING Ying. The Expression and Regulation of GSK-3β, CDK-5 and PP2A in Differentiated Neural Stem Cells of Rats. Life Sci J 2012;9(1):413-418] (ISSN:1097–8135).http://www.lifesciencesite.com. 61
Key words: neural stem cells; GSK-3β; CDK-5; PP2A; ginsenoside Rb1; Aβ25~35