Control the pos
Control the post harvest infection by Aspergillus spp. to Taify table grape using grape epiphytic bacteria
Abd El-Raheem Ramadan El-Shanshoury 1, 3*, Saleh Ali Bazaid 2, Yasser El-Halmouch1, 4 and Mohamed Waheed Eldin Ghafar 1, 5
1. Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 888, Taif 21974, Kingdom of Saudi Arabia
2. Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif 21974, Kingdom of Saudi Arabia
3. Department of Botany, Bacteriology Unit, Faculty of Science, Tanta University, Tanta 31527, Egypt
4. Department of Botany, Faculty of Science, Damanhour University, Damanhour 22511, Egypt
5. Department of Zoonoses, College of Veterinary Medicine, Cairo University, Cairo, Egypt
Abstract: This study aimed to control the common fungi causing post-harvest black rot of Taify table grape (Vitis vinefra L.) and to induce resistance against black Aspergilli. Six fungal genera belonging to Aspergillus,Penicillium, Trichoderma, Mucor, Rhizopus and Botrytis were isolated from contaminated grape samples, with different frequencies. The predominant fungal species belonged to the genus Aspergillus and the isolation frequency ranged between 2.40-18.66%. The most predominant black Aspergilli were identified to the molecular level based on 18S- rRNA, ITS1 and 5.8S-rRNA gene sequence as A. niger BAVSH1, A. parasiticusBAVSH4 and A. tubingensis BAVSH5. Forty one bacterial isolates were obtained from soil, meswak and the surface of grape fruits, and screened for antagonistic activity. In vitro dual microbial culture showed variable % inhibition of fungal growth values. Out of the active antagonistic bacteria, five epiphytic from grape fruits and one from meswak, being the most antagonistic were identified to the molecular level based on 16S rRNA gene sequence as Pseudomonas aeruginosa EBMSH1, P. aeruginosa EBVSH13, P. aeruginosa EBVSH14, P. aeruginosa EBVHSH17, Bacillus vallismortis EBHVSH28 and B. amyloliquefaciens EBHVSH29. The ability of living cells or crude cell extracts of the best three latter antagonistic bacteria to induce the resistance in intact- flooded grape fruits against black Aspergillus rot disease progress were demonstrated. Higher reduction or complete absences of disease incidence% and lesion diameter were obtained, compared to those induced by living bacteria and no treated control.
[El-Shanshoury AR, Bazaid SA, El-Halmouch Y, Ghafar M. Control the post harvest infection by Aspergillusspp. to Taify table grape using grape epiphytic bacteria. Life Sci J 2013; 10(1):1821-1636] (ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: Aspergillus spp.; Bacillus spp.; biological control; epiphytic bacteria; Pseudomonas sp.; post harvest