The Protective Effect of Ginger (Zingiber Officinale) Against Adriamycin- Induced Hepatotoxicity in Rats: Histological Study
Mohamed Atif Ahmed Said Ahmed
Anatomy Department, College of Medicine, Zagazig University, Egypt
Abstract: Aim: The present study aimed to show the histopathological and ultrastructural changes occurred in the rat liver post-application of adriamycin and to evaluate the possible protection of ginger against adriamycin induced hepatic toxicity. Material And Methods: Animals were divided into four groups. The first group was given saline orally. The second group was given ginger extract orally with a single daily dose 250 mg/kg b.wt. for two weeks. The third group was treated with adriamycin in six intraperitoneal injections (each containing 2.5 mg/kg/b.wt) over a period of two weeks. The fourth group was treated with adriamycin at the same dose level as those of group III and by oral administration of ginger extract (250 mg/kg/b.wt) daily for two weeks. Livers specimens from all groups were examined by light and electron microscopies. Results: Adriamycin treated group revealed destruction of the hepatic cords, cytoplasmic vacuolization, cellular degeneration, pyknotic and atrophic nuclei, damaged mitochondria and accumulation of lipid droplets in the hepatocytes and deposition of a collagen-like fibrous material in the blood sinusoids and space of Disse with dilated intercellular spaces. Dilated blood sinusoids and dilated bile canaliculi with atrophy of its microvilli were also noticed. Damaged Kupffer cells with vacuoles were prominent. The hepatotoxic effect of adriamycin was ameliorated with partial disappearance of pathological hepatic damage when treatment was combined with ginger. The results of the present work indicated that ginger had protective effect against liver damage induced by adriamycin.Conclusion: The present results provide in vivo evidence of direct chemotherapeutic hepatotoxicity caused by adriamycin. Appropriate protective measures as ginger must be applied with anticancer treatment for improving liver structure. The results can further suggest the possible use of ginger against oxidative stress induced organ toxicities.
[Mohamed Atif Ahmed Said Ahmed. The Protective Effect of Ginger (Zingiber Officinale) Against Adriamycin- Induced Hepatotoxicity in Rats: Histological Study. Life Sci J 2013;10(1):1412-1422] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key words: Ginger – adriamycin – hepatotoxicity – protection – histology- ultrastructure - liver.