Defensive Interaction of Different Lycopersicum esculentum cvs Infected by Tomato Bushy Stunt Virus
Elsayed Elsayed Hafez 1, Amany S. Youssef 2, Ghada S. Ali 2, Faiza A. Fattouh 2
1. Mubarak City for Scientific Research and Technology Applications, Arid Lands Research and Development Institute, New Borg El-Arab, Alexandria 21934, Egypt
2. Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21934, Egypt
Abstract: The Tomato bushy stunt virus (TBSV) P19 is one of a class of plant and animal virus proteins that are known to interfere with host defense mechanism, referred to as RNA silencing. Such proteins target foreign (virus and retrotransposon) RNAs in a sequence specific manner for degradation. Tomato, Lycopersicumesculentum L; Elisa, Super Strain-B (Super) and GS cvs were inoculated with the Tomato Bushy Stunt Virus (TBSVEgh). The P19 gene sequence was determined from both TBSVgh pure virus preparation and from virus inoculated tomato cultivars tissue. The sequence of the viral P19 gene showed similarity with P19 gene of Western United States TBSV with 98% identity. Differential display polymerase chain reaction (DD-PCR) was used for studying the up- and down-regulated genes in the three examined cultivars. High expression of Actin genes were observed in inoculated plants. The expression of plant defense genes was higher in L. esculentumMill Elisa cultivar than cultivar GS. Results indicated that some suspected defense genes, against TBSV Egh, are de novo genes.
[Hafez EE, Youssef AS, Ali GS, Fattouh FA. Defensive Interaction of Different Lycopersicum esculentum cvsInfected by Tomato Bushy Stunt Virus. Life Sci J 2013;10(1):1786-1794] (ISSN:1097-8135).http:www.lifesciencesite.com.
Keywords: Tomato bushy stunt virus; P19 gene; plant defense system; actin genes; differential display