Experimental Comparative Study of the Possible Effect of Panax Ginseng and Fish Code Oil against Acetaminophen Induced Hepatotoxicity
Wesam A.ElslamA.Elwahab1, Heba Gamal Abd El-Aziz2 and Mona A.A. Arafa3
1 Department of Forensic Medicine and Clinical Toxicology - Faculty of Medicine -Al-Azhar University
2 Department of Biochemistry- Faculty of Pharmacy-Al-Azhar University
3 Department of Anatomy- Faculty of Medicine -Al-Azhar University
Abstract: Background: Hepatic damage due to drugs, xenobiotics and environmental pollutants has been a serious concern worldwide. Acetaminophen is widely used as an analgesic and antipyretic drug, which can cause hepatic injury when given in high doses. Panax ginseng (Chinese medicinal herb) and Fish code oil with antioxidant properties would attenuate the intensity of the oxidative stress often involved in the pathogenesis of various diseases and have been studied in a variety of clinical conditions and found to protect against cellular damage. The objective of the present study was to study and compare the possible effect of ginseng and fish code oil against acetaminophen induced hepatotoxicity.Methods:36 rats were divided into six equal groups (n = 6): first group (healthy control), second group (positive control group), they were given a single oral dose of acetaminophen (500 mg/kg), the third and fourth groups were given Panax ginseng (300 mg/kg) and fish oil (4 ml/kg) as protection for 7 consecutive days prior to single oral dose of acetaminophen (500 mg/Kg), the fifth and sixth groups were first taken single oral dose of acetaminophen (500 mg/Kg) and after 3 days the animals were treated with ginseng and fish code oil for 7 days (300mg/Kg and 4 ml/Kg, respectively). Blood was taken from the orbital sinus of the rats for biochemical tests serum transaminases (ALT, AST), Alkaline phosphatase (ALP), Total protein (T.P) and Albumin (Alb.) and at the same time liver was removed and kept in 10% formalin solution for histological analysis. All histological sections were subjected to morphometric study, pathological evaluation followed by statistical analysis.Results: The results showed that the acute elevation of serum ALT,AST, ALP and the acute reduction of T.P and Alb. due to acetaminophen induced hepatotoxicity were significantly corrected in the groups receiving Panax ginseng and fish code oil. Necrosis of liver significantly decreased according to histopathologic observation, and that fish code oil was significantly more effective against acetaminophen induced hepatotoxicity when compared with ginseng.Conclusion: It is concluded that both Panax ginseng and fishcode oil have protective and treating effect on hepatotoxicity induced by acetaminophen and that fish code oil has better effect than Panax ginseng.
[Wesam A. Elslam A. Elwahab, Heba Gamal Abd El-Aziz and Mona A.A. Arafa. Experimental Comparative Study of the Possible Effect of Fish Code Oil and Panax Ginseng against Acetaminophen Induced Hepatotoxicity. J Am Sci 2013;9(6):234-244]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 27
Key words: acetaminophen, ginseng, fish code oil, hepatotoxicity Full Text 27