Oral and dermal
Oral and dermal exposure of chlorpyrifos and cypermethrin mixture induced cytogenetic, histopathological damage and oxidative stress in rats
1 Mammalian Toxicology Department, Central Agricultural Pesticides Lab. (CAPL), Agricultural Research Center, Ministry of Agriculture, Egypt.
2Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Bani Sweif University, Egypt
Corresponding author: firstname.lastname@example.org
Abstract: Present study is aimed to estimate the risk and the hazard of oral and dermal exposures of chlorpyrifos and cypermithrin mixture which are commonly used in Egypt. And to evaluate which type of exposure is more toxic or dangerous than other. Subacute toxicity of repeated 28 days in male white rats was assessed. The study was included some toxicological parameters ranged from cytogenetic, histopathological and oxidative stress effect. Eighty Wistar strain rats (weighing 140-160g) were randomly divided into four main groups (a, b, c and d) which were further subdivided into subgroups. Each of group (a) and (b) consists of 5 subgroups and were used to determine the oral and dermal LD50. Each of group (c) and (d) consists of 3 subgroups and were used in oral and dermal treatments respectively. The first subgroups of (c) and (d) were kept as control. Rats of two subgroups (c) treated orally by gavage 1/20 and 1/30 of LD50. Rats of two subgroups (d) treated dermally by 1/30 and 1/50 of LD50. The results revealed that, the pesticides mixture induced neurotoxicity, genotoxicity, severe histological changes. Also resulted in lipid peroxidation, inhibition in the activities of antioxidant enzymes (Cat) and reduced the glutathione contents. In addition the results showed the dermal treatments were more toxic and hazard than oral treatments. So, these mixtures of pesticides when present together may induce bio-activation sites resulting in the increase of the observed toxicity of these pesticides mixture compared with the toxicity of the individual alone. Finally, the results strongly impose the need to more detailed testing of the toxicity of mixture exposure than to one individual.
[Mohamed A. Noaishi, Amr A. Abd Allah and Mostafa M.M. Afify Oral and dermal exposure of chlorpyrifos and cypermethrin mixture induced cytogenetic, histopathological damage and oxidative stress in rats. J Am Sci 2013;9(3):56-65]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 9
Key words: chlorpyrifos, cypermethrin, mixture, Rats, chromosomal aberrations, Histopathology, oxidative stress. Full Text 9