Colchicine Induction of Polyploidy in Egyptian Clover Genotypes
Abd El-Naby, Zeinab, M1.*, Nabila A. Mohamed1, Khalid H. Radwan2, andDina A. El-Khishin2
1Field Crops Research Institute (FCRI). Agricultural Research Center (ARC) 9, Gamma St., Giza-12619, Giza, Egypt; 2Agricultural Genetic Engineering Research Institute (AGERI). Agricultural Research Center (ARC) 9, Gamma St., Giza-12619, Giza, Egypt. firstname.lastname@example.org
Abstract: Induction of polyploidy and chromosome comparison of eight berseem clover (Trifolium alexandrinum L.) genotypes were performed. Different colchicine concentrations were applied to seeds and seedlings using two methods: immersion and sprinkling. Both methods were successful in polyploidy induction. Karyotyping of F1 seeds andtheir parents were examined for polyploidy. Chromosome number was determined in root-tip cells 2-3d old from seed sowing. Tetraploids were evident in all populations (2n= 4X=32), whereas diploid parents had (2n=2X=16) chromosomes. Interactions between genotypes and colchicine treatments recorded high significant differences of no. inflorescences plant-1, 1000-seed weight, no. floret inflorescence-1 and no. seeds floret-1 with significant differences of seed weight g m-2, seed setting% and mortality%. Higher variation in leaflet shape and number, inflorescence size and shape and floret size was viewed over all colchicine treatments in tetraploid plants compared with the control. High positive significant correlation coefficient between number of seeds per floret and number of florets plant-1 (r=0.853**) and seed setting% (r=0.943**), mortality% was negatively correlated with seed setting% (-0.795**). Positive significant correlation was found between 1000-seed weight and number of florets plant-1 (r=0.711*) and seed setting% (r=0.744*). Polyploidy induction resulted in taller plants, faster re-growth after cutting, increased tillering and branching plant-1, increases in 1000 seed weight and increased seed yield m-2. This methodology can be used in plant breeding programs to increase forage production efficiency and select for more superior adaptive genotypes.
[Abd El-Naby, Zeinab, M, Nabila A. Mohamed, Khalid H. Radwan, and Dina A. El-Khishin. Colchicine Induction of Polyploidy in Egyptian Clover Genotypes. J Am Sci 2012;8(10):221-227].(ISSN: 1545-1003).http://www.jofamericanscience.org. 33
Key words: Berseem clover, Colchicine, Chromosomes, Correlation, Ploidy. Full Text 33