Effect of Lactoba
Effect of Lactobacillus acidophilus versus Bifidobacterium bifidum on clinical and
immunological responses of children with acute diarrhea
Michael N. Agban1, Noha A. Afifi1 and Nermeen S. Tawfeek2
1Department of Microbiology and Immunology, Faculty of medicine, Assiut University, Egypt.
2 Pediatric registrar in Al Eman hospital, Assuit,Egypt, and Al Sabah hospital, Kuwait. (E.mail:firstname.lastname@example.org
Abstract: Aim: To determine the value of Lactobacillus acidophilus versus Bifidobacterium bifidum in shortening the duration of acute diarrhea treatment in children. Also to measure the immune response to such treatment by measuring serum total IgA, IL-10 and TNF-α. Methods: Ninety Five children suffering from acute diarrhea were examined microbiologically to detect the causative organism, and then were divided into four groups: Group1; received L.acidophilus, Group 2; received B. bifidum, Group III; received both probiotics, and Group IV; (control group) received oral rehydration therapy. Only those patients suffering from bacterial, fungal or parasitic infections received antibacterial, antifungal or antiparasitic therapy respectively. Serum IgA, IL10 and TNF-α were measured in all patients using ELISA.Results: There was a significant decrease in the duration and frequency of diarrhea in groups I, II and III compared to group IV. Serum levels of IgA, TNF-α and IL-10 after treatment were significantly higher in groups I, II and III compared to group IV with no significant difference between group I and II, whereas group III showed higher levels of TNF-α and IL-10. However, in parasitic infections the levels of IgA, TNF-α and IL-10 were only increased in group III versus group IV. Moreover, fungal infections were not associated with change in IgA and TNF-α in any of the groups with probiotic treatment compared to the control group. A significant positive correlation between serums TNF-α and IL-10 was also observed in all the groups with probiotic treatment but not in group IV. Conclusions: Both L.acidophilus andB.bifidum are exhibiting antimicrobial activities in pediatric viral and bacterial diarrhea and their combined use may be useful in parasitic infections. A great advantage of the selected probiotics is their ability to induce IgA and TNF-alpha with their anti-infectious effects and IL-10 which down regulates pro-inflammatory cytokines secretion, thereby maintaining the delicate balance between necessary and excessive defense mechanisms.
[Michael N. Agban, Noha A. Afifi and Nermeen S. Tawfeek. Effect of Lactobacillus acidophilus versusBifidobacterium bifidum on clinical and immunological responses of children with acute diarrhea. J Am Sci 2012;8(10):772-781]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 104
Keywords: probiotics, L. acidophilus, B. bifidum, diarrhea, IgA, TNF, IL-10. Full Text 104