Evaluation of Pro
Evaluation of Probiotic Bacteria Exo-polysaccharides on Immune System.
Sh.M. Selim 1-3; Gehan F. Galal 1-3; Sharaf.M.S2-3; Mona S. Zayed 3.
1Biotechnology Department Faculty of Science and Education- Al-Khurmah, Taif University; KSA.
2 Biotechnology Department Faculty of Science, Taif University; KSA.
3Microbiology Department, Faculty of Agriculture, Ain Shams University Cairo, Egypt.
Abstract: Five Lactic acid bacteria (Lactobacillus rhamnosus; Lactobacillus helveticus; Lactobacillus acidophilus; Lactococcus cremoris; and Lactococcus lactis) were isolated from some Dairy products collected from Kingdom of Saudi Arabia (KSA) markets. Biomass and Exo-polysaccharides (EPS) production by Lactic acid bacteria (LAB) were studied under batch culture conditions using shake flasks. The effect of media type (M17 –MRS);Temperature (32,37& 42 ᵒC); pH levels (5.5,6.5&7.0) and different carbon sources (fructose- sucrose) were investigated to maximize the biomass and Exo- polysaccharides production. The probiotic bacteria exo- polysaccharides obtained immunologically were evaluated using Ten Swiss albino strain mice's. The highest yield of biomass and EPS production were obtained with Lactobacillus rhamnosus grown on MRS medium with pH7.0, glucose as a sole carbon source at 37 ᵒ C or 42 ᵒC for 48 hours. The EPS produced byLactobacillus rhamnosus were dosage orally with 108 UFC /ml / mice /day for 21 days to experimental group. The percentage of neutrophils (NU%), Lymphocytes (LY%), basophiles (BA%) and eosinophils (EO%) increased significantly (P≤ 0.05) in the experimental group (G1) compared to control group (G2). Immunoglobulin IgA showed a significant increase (P≤ 0.05) in the experimental group. However, no significant differences between G1 and G2 in immunoglobulin IgG. So. The present study concluded that Lactobacillus sp. had a power to activate the immune system.
[Sh.M. Selim; Gehan F. Galal; Sharaf. M.S; Mona S. Zayed. Evaluation of probiotic bacteria exo-polysaccharides on Immune system. Life Sci J 2013;10(1):2719-2725] (ISSN:1097-8135).http://www.lifesciencesite.com.
Key words: Probiotic bacteria, Lactobacillus sp., Exo-polysaccharides Fermentation, Immune system.