Jun Tan1, Juan Li2, Chengbiao Lu2, Tingtong Yang3, Yanyan Han2, Jianke Xu4, Yuming Xu*
3Department of Pathology, Xinxiang Medical University, Xinxiang, Henan, China
Abstract: This study was to evaluate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the proliferation of endogenous neural stem cells (NSCs) after cerebral ischemia-reperfusion injury (I/R injury) in rats. The animal model of I/R injury was established by middle cerebral artery occlusion (MCAO) method. Rats were divided into 3 groups: Sham operation group, ischemia-reperfusion group (I/R group) and I/R + rhG-CSF group. NSCs proliferation activity in hippocampus was evaluated using BrdU (5-bromo-2’-deoxyuridine) cell proliferation assay. The expression of two key molecules, Wnt1 and β-catenin, which play an important role in the signal transduction pathway of mediating NSCs proliferation, was determined by RT-PCR and Western Blot. Our study showed that the number of BrdU positive cells in I/R group significantly increased after 3d, and reached the peak after 7d, compared with the sham group. When the rats were treated with rhG-CSF (30 μg/kg/d), the NSCs proliferation activity were enhanced significantly, compared with the other two groups. Furthermore，the mRNA and protein expression levels of Wnt1 and β-catenin also were significantly higher in I/R + rhG-CSF group than that in the other two groups. These findings demonstrated that ischemic brain damage can induce the NSCs proliferation in the hippocampal dentate gyrus of adult rats and suggested that rhG-CSF could be used as an potential agent to repair the cerebral injury induced by ischemia-reperfusion.
[Jun Tan, Juan Li, Chengbiao Lu, Tingtong Yang, Yanyan Han, Jianke Xu, Yuming Xu. Granulocyte colony-stimulating factor enhances the proliferation of endogenous neural stem cell after cerebral ischemia-reperfusion injury in rats. Life Sci J 2013:10(1):3155-3459]. (ISSN: 1097-8135). http://www.lifesciencesite.com.
Keywords: Neural stem cells, Proliferation, I/R injury, rhG - CSF, BrdU.