Assessment of Afla
Assessment of Aflatoxin M1 Residues in Raw Cow Milk at Al- Riyadh Area with Reference to Some Detoxification Applications
Yosef, T.A.1*; Al- Julaifi, M.Z.2; Salah-El-Dein W.M.3 and AL-Rizqi, A.M.2
1Dept. of Forensic Med. and Toxicology, Fac. of Vet. Med., Kafrelshiekh Univ., 33516, Egypt.
2Toxicology lab. Management of Vet. Laboratories, Min. of Agric, Riyadh, 11418, KSA.
3Animal Health Research Inst., Dept. of Food Hygiene, Zagazig Provincial Lab., 44516, Egypt.
Abstract: This study was carried out to evaluate the levels of aflatoxin M1 (AFM1) in sixty raw cow milk samples collected from different farms at Al- Riyadh area, Saudi Arabia, besides reviewing the reduction effects of some detoxification methods on it. Results of the field study revealed that the mean concentration of AFM1 was 0.185 ±0.0181 ppb. On the other hand, 43 (71.7%), out of 60 examined samples, contained AFM1 residues in levels exceeded the EU maximum limit for raw milk (0.05 μg/l). Meanwhile 32 (53.3%), out of 60 samples, surpassed the Gulf maximum limit for raw milk (0.2 μg/l). For experimental study, negative milk samples for AFM1 were mixed and divided into 4 main groups which inoculated with 10, 5, 2.5 and 1.25 µg/l AFM1 standard respectively. Each group subdivided into 4 subgroups of 5 samples (100 ml each). The 1st subgroup let as control, the 2nd subgroup undergo pasteurization at 65°C for 30 minutes following by sudden cooling at 4°C, the 3rd subgroup treated by boiling at about 100°C for 10 minutes; while, the 4th one exposed to microwave radiation for 2 minutes in microwave oven at high energy level. The obtained results exhibited a significant reduction in AFM1 concentrations by all treatment methods comparing with the actual positive control levels. The reduction rate were ranked as follow: microwave radiation exposure (52.08%) > boiling treatment (23.93%) > pasteurization treatment (12.90%). Accordingly, microwave irradiation of AFM1 contaminated cow milk may be valuable to reduce its levels and subsequently minimize its hazardous on the public health.
[Yosef, T.A.; Al- Julaifi, M.Z.; Salah-El-Dein W.M. and AL-Rizqi, A.M. Assessment of Aflatoxin M1 Residues in Raw Cow Milk at Al- Riyadh Area with Reference to Some Detoxification Applications. Life Sci J 2013;10(1):3365-3369 ]. (ISSN: 1097-8135). http://www.lifesciencesite.com.
Key words: Aflatoxin M1; Milk; Boiling; Pasteurization; Microwave.