Assessment of DNA
Assessment of DNA Damage in Testes from Young Wistar Male Rat Treated with Monosodium Glutamate
Ismail, N. H.
Zoology Dept., Faculty of Girls for Art Sciences and Education, Ain Shams University
Abstract: Monosodium glutamate (MSG), the sodium salt of glutamate, is a flavor enhancing food additive that may be present in packaged food without appearing on the label. This could lead to inadvertent consumption of monosodium glutamate in high concentrations. The study investigated the effects of MSG on testes of young male Wistar rats, by daily oral exposure to 8g /kg b.wt. of monosodium glutamate for 90 days. Wistar rats (n=24) of average weight of 65- 80g were randomly assigned into two groups A, and B in each group (n=12). The control group (A) received distilled water. The treatment group ( B) were given oral doses each was 8g/kg body weight of monosodium glutamate by gavage on daily doses for 90 days. Rats were sacrificed on day 90 of the experiment. The testes were carefully dissected out and quickly fixed in Bouin`s for routine histological procedures. Results :The histological changes in the testes of rats due to the administrated MSG were mainly alterations of the seminiferous tubules which included atrophied malformed profiles ,appearance of hyaline material in the lumina of the seminiferous tubules and interstitial connective tissues .In addition sloughing and exfloliation of spermatocytes , spermatids, and immature germ cells appeared into the lumen of the seminiferous tubules .Many cells of the different types of spermatogenesis appeared with pyknotic nuclei and necrotic cells ,and dilated congested inter tubular blood vessels. Vacuolations were also observed between the inner cells of seminiferous tubules in the treated group. Increase in sperm shape abnormality was recorded . Furthermore, the alkaline Comet Assay showed significantly increased tail moment in testes cells of animals treated with MSG compared to control group. Conclusion: MSG may have some deleterious effects on the testes of Wister rats and by extension may contribute to the causes of male infertility. Thus, it is important to reconsider the usage of MSG as a flavor enhancer.
[Ismail, N. H. Assessment of DNA Damage in Testes from Young Wistar Male Rat Treated with Monosoddium Glutamate Using the Comet Assay. L Sci J 2012;9(1):930-939]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 136
Key words: DNA Damage; Monosodium Glutamate; Male Rat. Full Text 136