Attenuation of spe
Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene
Wang Peng 1, Zhang Zhenxiang 1*, Kan Quancheng 2, Yu Zujiang 2, Li Ling 3, Pan Xue 1 , He Hongjun 1, Feng Ting 1, Li Xiangnan 1, Jiang Li-li 1 , Zhai Guangyu 1 and Cui Guanglin 4
1. Nursing College, Zhengzhou University, Zhengzhou, Henan 450052, China
2. Clinical Pharmacology Base, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China
3. Department of Palliative Care and Hospice Care, the Ninth People’s Hospital of Zhengzhou, Zhengzhou, Henan 450053, China 4. Department of Gastroenterology, Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China. firstname.lastname@example.org
Abstract: Hepatocyte transplantation (HT) has been proposed as an alternative therapy to orthotopic liver transplantation (OLT) for patients with acute liver failure and metabolic disorders and hepatocytes are attractive targets for gene therapy. Adenovirus vector is considered a safe and efficient way to introduce foreign genes into several kinds of cells and is widely used in the various fields of gene therapy. But the response of host immune systems against gene products expressed by genetically modified cells is a major obstacle to successful gene therapy. Major histocompatibility complex (MHC) class I antigen presenting pathway is very important in acute allograft rejection and blocking MHC I antigen expression is becoming a research hotspot of inducting immune tolerance. Infected cell protein 47 (ICP47) expressed by herpes simplex virus type 1 (HSV-1), inhibits MHC I antigen presentation pathway by binding to host transporter associated with antigen presentation (TAP), and thereby attenuates of specific cytotoxic T lymphocytes（CTL）responses by virus-infected cells and evades the host immune clearance. This subject was designed to construct a recombinant adenovirus expressing His-tag-ICP47 fusion protein to investigate further the role of ICP47 in the elimination of transgene expression.
[Wang Peng, Zhang Zhenxiang, Kan Quancheng, Yu Zujiang, Li Ling, Pan Xue, He Hongjun, Feng Ting, Li Xiangnan, Jiang Li-li, Zhai Guangyu and Cui Guanglin. Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene. Life Sci J2013;10(1):2069-2074] (ISSN:1097-8135). http://www.lifesciencesite.com.
Keywords: recombinant adenovirus; His-tag-ICP47 fusion gene; cytotoxic T lymphocytes.