Effect of GLP-1 on
Effect of GLP-1 on after Experimental Ischemic Reperfusion Injury in Rats
Hany El Sebaee1; Maged Haroum1 and Rehab Ahmed Mohammed1 Ahmed Soliman2
Departments of 1Physiolog and 2Pathology, Faculty of Medicine, Kasr Al-Aini Cairo University Egypt.
Abstract: Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted by L-cells of small intestine in response to nutrient ingestion. Although the major physiological function of GLP-1 appears to be in relation to glycaemic control, there is growing evidence to suggest that it may also play an important role in the cardiovascular system. GLP-1 receptors (GLP-1Rs) are expressed in the heart and vasculature of both rodents and humans, and recent studies have demonstrated that GLP-1R agonists have wide-ranging cardiovascular actions, such as modulation of heart rate, blood pressure, vascular tone and myocardial contractility. In this study the cardiac effect of native GLP-1 after experimental induction of ischemia was studied. Fifty rats were used in this study. Their weight ranged from 200-250 grams. Rats were anesthetized and the hearts were excised. The hearts were mounted on a Langendorff perfusion system and a retrograde perfusion was started within 3min of the heart excision. After 30 min of stabilization, the following groups were defined: (1) Control group (group1a): consists of 10 rats, no ischemia, the flow was continuous for 2 hours. (Sham operation). In the other groups the flow was turned off for 35 min to elicit global ischemia and reperfusion was continues for 120min. (2) Control group (group1b): consists of 10 rats, no pharmacological agents were added during the first 15min of reperfusion. (3) DPP4 inhibitor group (group2): consists of 10 rats, sitagliptin 20mg/l was added during the first 15min of reperfusion. (4) GLP-1group (group3): consists of 10 rats, GLP-1 (0.3nM/l) + sitagliptin 20mg/l were added during the first 15min of reperfusion. (5) GLP-1 high dose group (group4):consists of 10 rats, GLP-1(10.3nM) + sitagliptin 20mg/l were added during the first 15min of reperfusion. The following parameters were measured at the end of stabilization period (reading1) and at the end of reperfusion (reading2) 1- Heart rate / min. 2-Left ventricular developed pressure/mmHg =left ventricular systolic-diastolic pressure. 3-Rate pressure product/mmHg/min =HR × LVDP and 4-Maximum rate of pressure rise ΔP/ΔT /mmHg/sec. Histopathological studies of sections from the 5 studied groups were performed usingHematoxylin and Eosin.
[Hany El Sebaee; Maged Haroum; Rehab Ahmed Mohammed and Ahmed Soliman. Effect of GLP-1 on after Experimental Ischemic Reperfusion Injury in Rats. Life Sci J 2013;10(1):3426-3437]. (ISSN: 1097-8135).http://www.lifesciencesite.com. 434
Keywords: GLP-1, Sitagliptin, Mycaardial Infarction, Reperfusion Injury.