ZHOU Xiao-jun1 , LING Na2, Zhang Long1, LI Yong-hui1, YA Hui-yuan1*
1.Department of Life Sciences, Luoyang Normal University, Henan 471022, RP China
2. Life Science and Environment Science Research Center, Harbin University of Commerce, Harbin 150076, RP China
* Lynubio@126.com, email@example.com
Abstract: In order to investigate expression changes of genes related to chromosome remodeling induced by implantation with low-energy N+ beam in rice, the differentially expressed genes related to chromosome remodeling in treated samples (6×1017 N+/cm2), were screened using Agilent gene chip. The results showed that, in the treated samples, 1 out of 30 genes related to histone deacetylase was up-regulated. Expressions of 48 genes related to histone acetyltransferases (HATs), two SNF2-related domain containing proteins and one SWIM Zn-finger domain containing protein were up-regulated, one SWIB/MDM2 domain containing protein and one TAFII55 protein conserved region domain containing protein were down-regulated, expression of 3 GCN5-related N-acetyltransferase domain containing proteins were significantly different, in which one was up-regulated and two down-regulated. There are several conserved protein domains related to chromosome remodeling on the Microarray, Expressions of 2 of 38 bromodomainrelated genes were changed of which one is up-regulated and the other down-regulated. Expressions of 2 of 7 chromodomain related genes and 8 of 36 SET related genes were up-regulated. Besides, one expression of 14 LSD1 type domain containing protein was significantly up-regulated. These findings suggest that the expression changes of genes related to chromosome remodeling may regulate the expression of certain genes responding to ion implantation, and induce the biological effect of the rice implanted by ion beam.
[ZHOU Xiao-jun, LING Na, Zhang Long, LI Yong-hui, YA Hui-yuan. Expression Changes of Genes Related to Chromosome Remodeling Caused by Implantation with Low-energy N+ Beam in Rice. Life Sci J 2012;9(1):148-153] (ISSN:1097-8135). http://www.lifesciencesite.com. 22
Key words: Nitrogen ion beam; Rice; Chromosome remodeling; Genechip Full Text 22