Oral exposure to z
Oral exposure to zinc oxide nanoparticles induced oxidative damage, inflammation and genotoxicity in rat’s lung
Howaida Nounou 1, 2, Hala Attia 3, 4, Manal Shalaby 2, 5, Maha Arafah 6
1. Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria 21111, Egypt.Email: firstname.lastname@example.org
2. Department of Biochemistry, College of Science, King Saud University, Riyadh, 11421
3. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11495, KSA.
4. Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
5. Department of Biomedical technology, Institute of science biotechnology, City of scientific research and biotechnology. Alexandria 21111, Egypt. 6. Department of Pathology, College of Medicine, King Saud University, Riyadh 11495
Abstract: This study aimed to investigate the toxicity of oral ZnONPs on the rat's lung. Rats were divided into four groups each of ten rats. Groups I and II were treated orally with 40 and 100 mg/kg ZnONPs for 24 hrs. Groups III and IV received daily 40 and 100 mg/kg ZnONPs orally for 1 week. Ten untreated rats were used as control. Oral administration of ZnONPs induced eosinophilia and lymphocytes infiltration in the lungs in the four tested groups that peaked at 100 mg/kg/day for 1 week. Lipid peroxidation was significantly higher, while levels of reduced glutathione and catalase activity were lower in all ZnONPs-treated groups. Nitrite concentrations increased significantly in rat’s lung treated with 100 mg/kg for 24 hrs and in those treated with 40 and 100 mg/kg daily for 1 week. Levels of lung TNF-α were significantly higher after 24 hrs at high dose and after 1 week at both low and high doses. Interleukin-1β and pentraxin-3 levels were significantly increased at 1 week only at both low and high doses. There were lower levels of paraoxonase-1 and increased DNA damage in the four studied groups. Oral administration of ZnONPs induced lung injury possibly through oxidative stress, inflammatory response and DNA damage.
[Nounou H, Attia H, Shalaby M, Arafah M. Oral exposure to zinc oxide nanoparticles induced oxidative damage, inflammation and genotoxicity in rat’s lung. Life Sci J 2013;10(1):1969-1979] (ISSN:1097-8135).http://www.lifesciencesite.com.
Keywords: ZnONPs; oxidative stress markers; inflammatory markers; DNA damage; lungs.