Prevalence of Stre
Prevalence of Streptococcus pneumonia in patients diagnosed with pneumonia by culture and PCR
Seyedeh Maryam Mohammadi Nasab 1,3, Farshid Kafil zadeh 1, Leila Jafarpour 1, Majid amouei 2,Owrang Ilami3, Seyed Abdolmajid Khosravani3*
1. Department of Biology, jahrom Branch, Islamic Azad University, Jahroom, Iran
2. The ministry of health and Medical Education, Tehran, Iran
3. *Yasuj University of Medical Sciences, Yasuj, Iran
Abstract: Pneumonia is an acute infection of the lower respiratory tissues accompanied by signs andsymptoms and evidence of chest infiltration on x-ray or altered breath sounds on auscultation. Depending ontheir co-morbidities, residents may develop community-acquired, end-of-life or aspiration pneumonia.Streptococcus pneumonia (pneumococcus) is responsible for approximately two-thirds of bacterialcommunity-acquired pneumonia cases as well as the most deaths from pneumonia, particularly in the elderly. The risk is highest in individuals with diminished immune competence, smokers and those with chronicconditions including cardiovascular or pulmonary disease, and diabetes.The aim of this study was todetermine the bacterial etiology in adult patients with .pneumonia infection by implementing polymerasechain reaction. A total of 126 cases (45 were male and 81 were female; age range: 1-80 years, mean age: 44)who were admitted to our hospital and clinically diagnosed as pneumonia between November 2011 - June2012, were included in the study. Respiratory samples (sputum and blood) obtained from patients weresearched by PCR method (Sinagen companies) in terms of the presence of Streptococcus pneumonia. Thesamples were simultaneously inoculated onto 5% sheep blood agar. The bacterial etiology was identified in 63(50%) of 126 patients with .pneumonia and a total of 73 pathogens were detected. The leading organism wasS. pneumonia. It was concluded that PCR/RLBH method supplemented the determination of bacterialetiology in our study cases by S. pneumonia. Materials & Methods: After completing the questionnaire, patient tracking numbers of 126 sputum samples collected and then Gram stain, culture, and PCR wereperformed. Results: Of the126 samples, 35.7%were male and 64.3% female mean age was 44 years. Anumber of 45 patients were positive whereas the results for PCR were showed positive for 63 patients.Discussion: Theresult of this study has shown the importance of streptococcus pneumonia in the societyand because PCR is a fast method for detection of bacteria and can have good results in treating pneumoniabefore wasting time.
[Seyedeh Maryam mohamadi nasab, Farshid kafil zadeh, Leila jafarpour, Owrang Ilami, Asghar sharifi, andSeyed abdolmajid khosravani. Prevalence of Streptococcus pneumonia in patients diagnosed withpneumonia by culture and PCR. Life Sci J 2013;10(1):772-775]. (ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: streptococcus pneumonia, culture, PCR.