Study on Vancomyci
Study on Vancomycin-Resistant Staphylococcus aureus and Identification of VanA Gene in These Strains Isolated from Tabriz Shuhada Hospital Using E-Test and PCR Methods
Sughra Sheikh Muneeri1, Haedeh Mobaiyen2, Hamid Mirzaie3
1- M.S in Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2- Department of Microbiology, Faculty of Medical Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3- Department of Food Hygiene, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Abstract: Staphylococcus aureus is one of the main causes of hospital-acquired infections. Vancomycin is widely used in treating infections created by this organism which increases vancomycin-resistance and the spread of S. aureus strains with intermediate vancomycin resistance in different parts of the world has created major concerns about the clinical samples. Gathering data on the situation of drug resistance - especially vancomycin-resistance of these organisms not only is considered an effective measure to prevent hospital infections but also as one of research priorities. In this study, the frequency of vancomycin resistance and the presence of vanA gene are evaluated in S. aureus strains isolated from Tabriz Shohada hospital with PCR. 73 strains of Staphylococcus aureus were isolated from hospitalized patients in Tabriz Shohada Hospital during a period of eight months. All of strains were identified through the routine methods. The resistance to vancomycin was identified through agar disk diffusion method and MIC of vancomycin was measured through E-test. Also, in order to identify vanA genes, specific primers were applied. The frequency of intermediate vancomycin-resistant strains using agar disk diffusion method and E-test are % 26.02 and % 14.43, respectively. The results of PCR indicate that 1.3% of strains are identified as vancomycin-resistant. VanA- positive strains were far more resistant against antibiotics than vanA- negative. During the present study vanA gene was identified in only one strain of isolated S. aureus with PCR. Separation of vanA gene in isolated strains rings the alarm for authorities to control infection in the given hospital and other medical centers in Tabriz.
[Sughra Sheikh Muneeri, Haedeh Mobaiyen, Hamid Mirzaie. Study on Vancomycin-Resistant Staphylococcus aureus and Identification of VanA Gene in These Strains Isolated from Tabriz Shuhada Hospital Using E-Test and PCR Methods. Life Sci J 2013;10(1):748-752]. (ISSN: 1097-8135). http://www.lifesciencesite.com.
Key words: Staphylococcus aureus, Drug Resistance, vanA Gene, PCR, Vancomycin, E-test