The Effect of Omeg
The Effect of Omega- 3 Fatty Acids on the Age Related Changes in Submandibular Salivary Glands of Albino Rats
Dalia EL-Baz and Zainab A. Salem*
*Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
Abstract: Objective: The current study has been carried out to evaluate histologically, immunohistochemistry and statistically the effect of daily consumption of omega-3 on submandibular salivary gland of old aged rats. Material & methods: Eighteen male albino rats were used in this study. The rats were divided into three groups (6 rats/each): Group I: 4-6 months rats weighing 170-200 gm, represented the adult rats. Group II: 12-15 months rats weighing 250-300 gm, represented the old rats. Group III: : 12-15 months rats weighing 250-300 gm, represented the old rats, and receiving omega-3(60 mg/kg) once daily, for one month by intra- gastric intubation. All rats were sacrificed after 30 days. Soft tissue specimens were obtained from submandibular salivary gland at the floor of the mouth of the rats in all the studied groups. The sections were examined histologically with H&E, immunohistochemically using tumor necrosis factor alpha and the results were evaluated statistically. Results:Histopathological changes were observed in group II including; decrease in the overall size of the acini and granular convoluted tubules, an increase in the amount of fatty tissue, fibrous tissue and inflammatory infiltration. In group III, the acini and ducts appeared nearly normal and there was marked decrease in the inflammatory infiltration. Immunohistochemical examination revealed moderate expression of tumor necrosis factor alpha in group II, mild expression in group III and weak expression in group I. Conclusion: Omega-3 had significant effects in management of many age related changes of submandibular salivary gland.
[Dalia EL-Baz and Zainab A. Salem. The Effect of Omega- 3 Fatty Acids on the Age Related Changes in Submandibular Salivary Glands of Albino Rats. J Am Sci 2013;9(6):149-154]. (ISSN: 1545-1003).http://www.jofamericanscience.org. 17
Key words: Omega-3, Aging, Tumor necrosis factor alpha, Submandibular salivary gland. Full Text 17