Society for Endocrinology Annual Meeting 2003

Society for Endocrinology Annual Meeting 2003

London, UK
03 November 2003 - 05 November 2003
Society for Endocrinology

Endocrine Abstracts(2003)6DP14

Onset of insulin-resistant stage in a Type II diabetic rat model is associated with diminished levels of vascular endothelial growth factor (VEGF) and its receptors in the penis: implications in the pathogenesis of erectile dysfunction in diabetes

S Jesmin1, I Sakuma2, CN Mowa2, A Salah-eldin3, Y Hattori4& A Kitabatake1

1Cardiovascular Med, Hokkaido Univ Med Sch, Sapporo, Japan;2Neurobiol, NE Ohio Univ Coll Med, Rootstown, USA;3Environ. Med, Hokkaido Univ Environ Earth Sci Sch, Sapporo, Japan;4Pharmacol, Hokkaido Univ Med Sch, Sapporo, Japan.

The role of vascular endothelial growth factor (VEGF) in the pathogenesis of numerous complications associated with diabetes is well established. However, little is known of VEGF's role in erectile dysfunction (ED), a condition commonly experienced by diabetic men. We hypothesized that expression of VEGF, its receptors and its signaling pathway, Akt, may be altered in diabetic penile tissues and, consequently, induce expression of molecules involved in the development of ED in diabetes. The present study utilized Ostuka Long-Evans Fatty (OLETF) rats, animal models for Type II diabetes (20 weeks of age), to determine alterations, if any, in levels of VEGF, its receptors, Akt, nitric oxide synthase isoforms, and apoptosis-related molecules in the penis using immunohistochemistry, Western blotting, in situ hybridization, and real-time quantitative PCR analyses. Furthermore, penile sections were also tested for apoptosis by Tdt-mediated dUTP Nick End Labeling (TUNEL) assay. Levels of VEGF, its receptors and Akt were markedly reduced in the penises of OLEFT rats, and those of endothelial and neuronal nitric oxide synthase were expressed less abundantly. Furthermore, while anti-apoptotic markers, Bcl-2 and phosphorylated Bad, were down-regulated, pro-apoptotic markers, active caspases-3 and Bax, were up-regulated, as evidenced by the appearance of apoptotic cells in the penile tissues of OLETF rats. We propose that disruption of VEGF signaling system in the penis may play a role in the patho-physiology/genesis of diabetic ED.

Endocrine Abstracts(2003)6DP14

المواد الدراسيه Courses

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الساعات المكتبية

الأحـد 10-2 * الأثنين 12-3 * الثلاثاء 11-2

أعلانات : أختبــــارااااااات

First Mid-Term Examination Schedule - Second Semester 1435-1436



Course Name

Code & Number


13 / 5 / 1436

Medical Genetics

MDL 322


14 / 5 / 1436


MDL 211

MDL 242


18 / 5 / 1436


MDL 311

MDL 241


19 / 5 / 1436

Electron Microscope

MDL 314

MDL 362

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Second Mid-Term Examination Schedule Second Semester 1435-1436

مقالاتي: انت دمك خفيف

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التقويم : اليوم والساعة

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