Genetic
Genetic characterization of Pseudomonas aeruginosa isolated
from contact lenses and other sources by RAPD analysis
Salha HM Al-Zahrani*, Nariman AH
Aly and Maha A Al-Harbi
Department of Biology, Faculty of Science, King Abdulaziz University, Saudi
Arabia
Corresponding author: [email protected]
Abstract: Pseudomonas aeruginosa (24 isolates) collected from
contact lens storage cases, contact
lenses and contact lenses wearer in Saudi Arabia. PCR amplification of all
isolates revealed one fragment with 230 bp that represented 16S rDNA
gene. A total of 48 amplified DNA fragments
(from 3500 to 90 bp) were observed using three RAPD primers; B-01, B-11 and
B-14.Whereas, 42 fragments were polymorphic and the other 6 amplified fragments
were commonly detected among all Pa isolates. The three primers showed a mean
polymorphism of 87%, whereas, the polymorphic % B-01 primer was
higher (89%) followed by primer B-11 and B-14 with 88 and 83%, respectively. The constructed UPGMA dendrogram showed
two main clusters; the first included 10 isolates with a medium bootstrap 83% ,the second included the remaining
14 isolates with two sub-clusters. The first sub-cluster divided to two
branches; the first contained isolates 21 & 23 and the second was divided
more to two sub-branches. The second sub-cluster was divided to isolate 2 that
occupied unique branch and two other branches that divided again to several
sub-branches. Isolate 8 revealed most high similarity with isolate 7(77%)
followed by isolates 18 and 17 (74%). Isolates 3 & 1 and 22 & 15showed
similar percentage (72%). isolates 7 and 3 displayed (70%). Conclusion: high
DNA polymorphism occurrence of amplified fragments &Variable genetic
similarities of Pa isolates (24)was noticed . The
fluctuation of genetic similarity percentages of each of the isolates with
others revealed the divergent genetic backgrounds of different serotypes.
[SALHA HM AL-ZAHRANI, NARIMAN AH ALY AND MAHA A AL-HARBI. Calculate & Analyze of Growth in Vicia
FabaL. Plant. Life Sci J 2012;9(1):835-843] (ISSN:1097-8135). http://www.lifesciencesite.com. 121
Key
words: Pseudomonas
aeruginosa isolates, contact lens and
contact lenses wearer, 16S rDNA gene, RAPD-PCR Full Text 121
