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Changes of superoxide dismutase and catalase activities in crucian carp（Carassius auratus）exposed to copper and recovery response
Hongxia Jiang1 . Hongmei Yang2. Xianghui Kong1* . Shuping Wang1. Huiyun Guo1
1College of Fisheries, Henan Normal University, Xinxiang, Henan 453007, China
2Editorial Board of Journal of Zhengzhou University, Zhengzhou, Henan 450001, China
Abstract: In this study, the effects of antioxidant enzyme parameters in the freshwater fish Carassius auratus to water-borne copper (Cu2+) exposure were assessed, and the reversibility of enzyme activities post-copper exposure were studied. The fish were exposed to different concentrations (0.05, 0.1, 0.2, 0.5 and 1.0 mg/L) of copper for 96 h, and then the 1.0 mg/L exposure group was transferred to the clean water (control water without the introduced copper) and exampled after 1, 5, 10, 15, and 30d to assess the recovery profile. Responses of the activities of superoxide dismutase (SOD) and catalase (CAT) in kidney, gill, spleen and brain to copper exposure and the recovery profile were investigated. As shown in the results, the changes of the two antioxidant enzymes activities in different organs of C. auratus exposed to copper are different, SOD and CAT activities in gill and spleen increased at low copper concentrations and decreased at high copperconcentrations. SOD and CAT activities in brain all decreased at various copper concentrations, while SODactivity in kiney increased at all copper concentrations. After removing 1.0 mg/L copper exposure, all theantioxidant enzyme activities recovered to the normal levels within 30 days, and SOD and CAT activities in gillnormalized in the fastest speed, while the both enzyme activities in kindey normalized in the slowest speed. SOD and CAT activities in brain of C. auratus are more sensitive to copper exposure, which can be used assensitive biomarkers to assess copper contamination in aquatic ecology.
[Jiang HX, Lei MY, Kong XH, Wang SP, Guo HY. Changes of superoxide dismutase and catalase activities in
crucian carp（Carassius auratus）exposed to copper and recovery response. Life Sci J 2013;10(1):3281-3288](ISSN: 109 7-8135). http://www.lifesciencesite.com.
Keywords: Copper exposure; antioxidant enzyme; recovery; Carassius auratus.
