Construc
Construction and Characterization of a Bacterial
Artificial Chromosome Library from the Huoyan Goose
Peng Fei Hu†, Xiang Chen
Li†, Xian Wei Chen, Wei Jun Guan, Yue Hui Ma
Institute of Animal
Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
† These authors contributed
equally to this work
[email protected] & [email protected]
Abstract: Huoyan
goose is a famous Chinese local breed for its egg productivity. In this study,
a bacterial artificial chromosome library of the Huoyan goose was successfully
established using the Hind III site of the vector pBeloBAC11, comprising of
115, 200 clones arrayed in 543, 384 well microplates , with an average insert
size of 102 kb and the content of the library was 11.4 genome equivalents,
which yielded a theoretical probability of 99.93% for isolating a particular
DNA sequence. BAC clones of the library were stable in the bacterial host for
at least 100 passages.
[Hu PF,
Li XC, Chen XW, Guan WJ, Ma YH. Construction
and Characterization of a Bacterial Artificial Chromosome Library from the
Huoyan Goose. Life Sci J 2013;10(1):375-378].
(ISSN: 1097-8135).http://www.lifesciencesite.com. 60
Keywords: Bacterial artificial chromosome library; Huoyan goose;
genomic DNA Full Text 60
