Effect o
Effect
of Irradiation on Uricase Produced by Two Strains of Asperigillus niger
Usama F. Ali and Zeinab M.
Ibrahim
Biological and Geological
Dept. Faculty of Education, Ain Shams University, Cairo, Egypt
Abstract: The
main purpose of this research is to screen the available local fungal strains
for their uricase producing ability, to select the most potent isolate and also
to optimize the culture conditions for maximum uricase production. Two strains
of Aspergillus niger; A. niger (Thom) Thom &
Raper 1945 which is thermotolerant and A. niger van Tieghem
1867 which is mesophilic were used during this study. Uric acid medium was the
best for uricase production (2.14 and 2.45 U/ml); sucrose and starch were the
best carbon sources (2.1 & 2.77 U / ml) while casein and peptone were the
best nitrogen source (4.86 and 3.16 U/ml) ; alkaline medium (pH 9.0 & 8.0)
were the best for the experimental fungi (4.42 and 5.27 U/ml); higher
temperatures (45º & 35ºC) were the best the experimental fungi (4.65 and
5.11 U/ml ) . Low dose of Gamma radiation increased the production of uricase
(0.5 & 0.3 KGy) gave the best results for uricase production (11.82 and
12.32 U/ml).
[Usama F. Ali and Zeinab M. Ibrahim. Effect of
Irradiation on Uricase Produced by Two Strains ofAsperigillus niger. Life Sci J 2013;10(1):962-967]. (ISSN:
1097-8135). http://www.lifesciencesite.com.
Keywords: Aspergillus niger – Uricase production – Gamma irradiation.
