Effects
Effects of Nigella
sativa Oil and Thymoquinone on Renal
Oxidative Stress and Apoptosis Rate in Streptozotocin-Diabetic Rats
Dalia A. Hafez
Nutrition and Food Science
Department, Faculty of Design and Home Economics, Taif University, Kingdom of
Saudi Arabia
Home Economics Department, Faculty of Education, Suez Canal University,
Egypt
Abstract: The
effects of Nigella sativa oil (NSO) and thymoquinone (TQ) on
diabetes, renal oxidative stress, lipid peroxidation and apoptosis rate were
investigated in streptozotocin (STZ)-diabetic rats. Forty five Sprague Dawley
rats were randomly distributed into 5 groups (n=9): healthy control; diabetic
control; diabetic given NSO (1ml/kg); diabetic given TQ (3 mg/kg) and diabetic
co-administered with NSO and TQ, orally for 4 weeks. Diabetes was induced by a
dose of STZ (50 mg/kg, i.p.) in fasted rats. Blood samples were collected for
biochemical analysis. Kidneys were removed for determination of tissue
antioxidant capacity, lipid peroxidation and apoptosis rate. The results showed
that oral co-administration of NSO and TQ significantly increased serum insulin
and decreased serum levels of glucose, glycosylated hemoglobin (HbA1c), urea,
creatinine and alkaline phosphatase in diabetic treated rats. Treatment with
NSO and TQ significantly increased activities of antioxidant enzymes (SOD, GPx
and CAT), increased serum level of reduced glutathione (GSH) and decreased
lipid peroxidation and apoptosis index. In conclusion, NSO and TQ exhibit good
antidiabetic and antioxidant effects; improve renal function and reduce both
malondialdehyde (MDA) and apoptosis rate in diabetic rats. Therefore, NSO and
TQ may be beneficial for diabetes mellitus associated with nephropathy due to
oxidative stress.
[Dalia A. Hafez. Effects of Nigella
sativa Oil and Thymoquinone on Renal
Oxidative Stress and Apoptosis Rate in Streptozotocin-Diabetic Rats. J
Am Sci 2013;9(3):327-333]. (ISSN: 1545-1003).http://www.jofamericanscience.org. 46
Keywords: Nigella sativa oil;
Thymoquinone, Apoptosis; Kidney function; Lipid; peroxidation; Antioxidant; Full Text 46
