Identifi
Identification and Some Probiotic Potential of Lactic
Acid Bacteria Isolated From Egyptian Camels Milk
Eman Hamed1 and
Aisha Elattar*2
1Department of Botany and Microbiology, Faculty of
Science, Alexandria University
2 Department of Dairy Sciences and Technology
Faculty of Agriculture, Alexandria University. Egypt.
Abstract: A
study was carried out to investigate the probiotic potential of isolated lactic
acid bacteria from camel’s row milk collected from Arabian camels (Camelus
dromedaries) in Egypt. Eleven gram positive, catalase negative isolates
were identified using API 20STREP identification system for the identification
of cocci isolates and API 50CHL for bacilli. Isolates were identified as Enterococcus
faecium (seven isolates),Enterococcus durans (one
isolate), Aerococcus viridians (one isolate), Lactococcus
lactis (one isolate) andLactobacillus plantarum (one
isolate). The probiotic potential of these isolates was investigated
using in vitroantagonistic tests
against Salmonella typhi ATCC 14028, E.coli ATCC
25922 and Vibrio fluvialis using agar spot test. All of the
isolates were proved to be effective against those pathogens. Isolate ES08 was
able to inhibit the growth of indicator pathogens with an average inhibition
zone of 3.3, 3.7 and 2.0 cm in diameter against Salmonella typhi, E.coli and Vibrio
fluvialis, respectively. All of the isolates showed resistance to stomach
pH (pH 3.0), tolerance against 0.3% bile salts concentration and none of the
isolates caused blood hemolysis. Isolate ES08 was farther identified by
sequencing their 16S rRNA encoding gene.
[Eman Hamed
and Aisha Elattar. Identification and Some Probiotic Potential of
Lactic Acid Bacteria Isolated From Egyptian Camels Milk. Life Sci J 2013;10(1):1952-1961]
(ISSN:1097-8135). http://www.lifesciencesite.com.
Key Words: Camels milk, Lactic Acid Bacteria, Probiotic potential.
