Molecula
Molecular
characterization of Cotugnia polycantha (Cestoda,
Cyclophyllidea, Davaineidae) infecting doves (Streptopelia senegalensis)
and pigeons (Columba livia Domestica) from Egypt
Sabry E. Ahmed
Department of
Zoology, Faculty of Science, Zagazig University. [email protected]
Abstract: The genomic DNA was extracted from cestode parasites, Cotugnia
polycantha from two different hosts, doves (Streptoplia
senegalensis) and pigeons (Columba livia domestica). The random
amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to
differentiate between C. polycantha infecting doves and
pigeons. Eight primers of arbitrary sequences were used in the PCR reactions.
The eight primers screened gave total amplified fragment markers 133. The
total number of unique bands was 25 and the highest polymorphism percentage
(63.63%) was obtained among the two specimens. Molecular analysis of the
present data, showed that C. polycantha infecting doves (S.
senegalensis) differs from that infecting pigeons (C. livia
domestica). So, C. polycantha infecting doves could
be namedC. polycantha streptopeli and C.
polycantha infecting pigeons, C. polycantha columbi.
[Sabry E. Ahmed. Molecular Characterization of Cotugnia
polycantha (Cestoda, Cyclophyllidea, Davaineidae) Infecting Doves (Streptopelia
senegalensis) and Pigeons (Columba livia Domestica) from
Egypt. Life Sci J 2012;9(1):295-301]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 42
Key Words: Doves – Pigeons – Cestode- Genomic DNA – DNA
fragments. Full Text 42
