Proteomi
Proteomic
Profiling and Protein Identification by 2-DE gel electrophoresis combined with
MALDI-TOF Mass Spectrometry in Rat hepatocyte Nucleus
Xianguang Yang1, 2,
Chunling Yan1, 2, Dongdong Sun1, 2, Xiaofang Geng1,
2, Yanjie Yang2, Cunshuan Xu1,2*
1. College of Life Science,
Henan Normal University, Xinxiang, Henan 453007, China
2. Co-constructing Key
Laboratory by Province and the MOST for Cell Differentiation Regulation,
Xinxiang, Henan 453007, China
E-mail: [email protected]
Abstract: More
and more proteomics research focusing on nucleoprotein. In this article, the
proteomic profiling of Rat hepatocyte nucleus was showed by 2-D PAGE and a
total of 204 kinds of nucleoproteins were identified by MALDI-TOF Mass
Spectrometry. Addition to housekeeping protein, low kurtosis transcription
factors were enriched and identified too. Ingenuity Pathway Analysis (IPA)
showed that these nucleoproteins were involved in the rat liver cell cycle
regulation, DNA replication, recombination and damage repair, cell assembly and
organization, cell development, nervous system development and function, cancer,
cardiovascular system development and function, cell death and survival, cell
morphology, Cell-To-Cell signaling and interaction. These results can provide
more information for a comprehensive understanding to the function of nucleus;
on the other hand, the results lay the foundation for the analysis of
nucleoproteins expression varieties and help to look for the biomarker of
disease diagnosis and therapies.
[Xianguang
Yang, Chunling Yan, Dongdong Sun, Xiaofang Geng, Yanjie Yang, Cunshuan Xu. Proteomic
Profiling and Protein Identification by 2-DE gel electrophoresis combined with
MALDI-TOF Mass Spectrometry in Rat hepatocyte Nucleus. Life Sci J 2013:10(1):3183-2191].
(ISSN: 1097-8135).http://www.lifesciencesite.com.
Keywords: Rat hepatocyte, Nucleoprotein, Proteomics, 2-D gel electrophoresis, MALDI-TOF MS.
