The expr
The expression of UDP-glucose pyrophosphorylase 2 following
acute myocardial infarction in rat
LuShun Zhang 1,
LinBo Gao 2, WeiBo Liang 1, YunLiu 1,
TianYi Chen 1 and Lin Zhang 1
1 Department of
Forensic Biology, West China School of Preclinical and Forensic Medicine,
Sichuan University, Chengdu, Sichuan 610041, China.
2 Laboratory of
Molecular and Translational Medicine, West China Institute of Women and
Children’s Health, West China Second University Hospital, Sichuan University,
Chengdu, Sichuan 610041, China
Abstract: Acute myocardial infarction (AMI) is
a major cause of sudden cardiac death (SCD) in patients with coronary and
ischemic heart diseases. However, the age of AMI estimation in SCD is not fully
known. The aim of this study was to investigate the expression of UDP-glucose
pyrophosphorylase 2 (UGP2) in AMI. A total of 71 Sprague–Dawley male rats were
divided into AMI group, sham-operated group and normal control group. After
surgery, the myocardium was harvest following 0 min, 15 min, 30 min, 60 min,
120 min, 240 min in AMI group and sham-operated group. The expression of UGP2
in myocardium of rats was detected using quantitative real-time PCR and Western
Blot. UGP2 mRNA and corresponding protein expression were
decreased after ligation 15 min, and significant differences were found in UGP2 mRNA
(after ligation from 30 min to 120 min) and UGP2 protein (after ligation from
30 min to 240 min) in the AMI zone compared with the control, respectively.
These findings suggest that UGP2 may be used as a potential biomarker for the
time estimation in AMI.
[LuShun Zhang, LinBo Gao, WeiBo Liang, YunLiu, TianYi Chen, Lin Zhang. The expression of UDP-glucose pyrophosphorylase 2 following acute myocardial infarction in rat. Life Sci J 2013;10(1):3299-3302] (ISSN:1097-8135). http://www.lifesciencesite.com.
Key words: UGP2; AMI; Age of AMI; SCD.
