Detection
Detection of Genotoxicity of Phenolic Antioxidants, Butylated hydroxyanisole and tert-butylhydroquinonein Multiple Mouse Organs by the Alkaline Comet Assay
Ramadan, A.M. Ali1, 2, *, Takayoshi Suzuki2
1Zoology Dept., College for Girls for Science, Arts and Education, Ain-Shams Univ., Heliopolis, Cairo, Egypt; 2Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158, Japan.[email protected]
Abstract: In this study we tested the genotoxicity of three widely used phenolic antioxidants, butylated hydroxyanisole (BHA) and tert-butylhydroquinone (t-BHQ) in multiple mouse organs using the alkaline comet assay. Tissue samples from four organs (stomach, liver, kidney and bone marrow) were collected from mice at 3 and 24 hrs post treatment with BHA (800 mg/kg) or t-BHQ (400 mg/kg) and examined for genotoxicity. The two compounds induced significant increase in DNA migration in a time dependant manner in specific organs. Extensive DNA damage was observed in stomach cells at 24 hrs post treatment in treatment groups. In addition to stomach, t-BHQ treatment induced significant increase in DNA migration in liver and kidney cells also. Although increased DNA damage was found in kidney cells of treatment groups at 3 hrs time point, at later time point it was persistent only in mice treated with tBHQ and in other treatment group (BHA) it appeared to be recovered with time. Evidently, bone marrow cells did not show genotoxicity in response to treatment with t-BHQ and BHA.
[Ramadan, A.M. Ali and Takayoshi Suzuki. Detection of Genotoxicity of Phenolic Antioxidants, Butylatedhydroxyanisole and tert-butylhydroquinonein Multiple Mouse Organs by the Alkaline Comet Assay. Life Sci J 2012;9(1):177-183]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 25
Key wards: BHA; t-BHQ; comet assay; mice; bone marrow; liver; kidney; stomach; ENU Full Text 25
