Comparativ
Comparative Molecular Analysis of Egyptian Strains of
Highly Pathogenic Avian Influenza (HPAI) Virus H5N1 Isolated During 2006 And
2007
Madbouly, H.M1;
Orkhan, M.H2; Nagwa El-Kholy2 and Hussein, A.S1.
1Department of Virology Faculty of Veterinary Medicine,
Beni-Suef University, Egypt
2World Health Organization National Influenza Center
(WHONIC), VACSERA, Egypt
Abstract:Isolated
strains of HPAI H5N1 during February 2006 from infected chicken located at
El-Qanater El-Khiria center, Qaluobia, Egypt were molecularly characterized and
compared with those isolated strains during January 2007 from the same
locality. The isolated strains from infected chicken during 2006 were closely
related to strain isolated from turkeys located in Turkey at 2005 (A/ty/Turkey/1/05) and
toA/Vietnam/1194/04 strain isolated from human cases of AI H5N1 and
induced higher case fatality in human in Vietnam. The phylogenetic analysis of
HA and NA genes of the isolated strains during 2006 revealed that, these
isolates are located in Eurasia- African lineage which contains AI H5N1 strains
from Egypt, Turkey, Romania, Iraq, Mongolia, Iran, Korea and Nigeria and
differed from those isolated in South-Eastern of Asia which contains Cambodia,
Thailand, and Vietnam Nam strains of AI H5N1. The phylogenetic analysis
of HA, NA, and M genes of Egyptian isolates of January 2007 from the same
locality showed minor antigenic variation from those of 2006 and located
in a new sub-clade from Egyptian strains of 2006 and all of them located
in Euro-African lineage. All 8 genes were closely related to the genes of
onther Egyptian H5N1 isolates from chicken, duck, and human. The sequence of NAs
did not possess any know oseltamivir resistance mutations. Also the M2
sequences did not possess amantadine resistance mutations.
[Madbouly, H.M; Orkhan, M.H; Nagwa El-Kholy and
Hussein, A.S. Comparative Molecular Analysis of Egyptian Strains of Highly
Pathogenic Avian Influenza (HPAI) Virus H5N1 Isolated During 2006 And 2007. J
Am Sci 2012;8(10):254-240]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 38
Key words: Egyptian strains - highly pathogenic- avian influenza
(HPAI) virus H5N1 Full Text 38
