Histologic
Histological and Immunohistochemical studies of the effect of vitamin C and Nigella sativa on the palate of albino mice’s offspring after cadmium exposure.
Shaymaa Hussein1; Mohamed El-Sakhawy1; Abdel-Aleem El-Saba1; Abd Rabou M.I.; Hany Sherif2and Abdel Razik Hashim2
1 Department of Cytology & Histology, Faculty of Vet. Medicine, Cairo University, Egypt.
2Department of Oral Biology, Faculty of Dental Medicine, Al-Azhar University, Egypt.
Abstract: Performance of the current study utilized a well elicted CD-1 albino mouse. 50 females divided into 5 groups, 10 females in each group separated from males for one month, all groups were get the drug doses twice a week for one month before gestation and during pregnancy until delivery. For breeding purposes each 2 females were mated with 1 male from 5 pm. until 9 am. at the following day. Female mice were classified into five groups. Group I (control group, female animals were injected intramuscularly with 0.5 ml of sterile normal saline); group II (dams were injected i.m. with 5 mg/kg body weight of cadmium chloride (Cd Cl2) dissolved in 0.5 ml of sterile normal saline); group III; (treated with Cd Cl2 as in group II, plus i.m. injection of 10 mg/ kg body weight of vitamin C. group IV(was injected i.m. with Cd Cl2 as in group II and given 50 mg/kg body weight of Nigella sativa orally) and group V(animals were administrated cadmium and vitamin C as in group III and Nigella sativa as same as in group IV). After delivery, heads of pups were decapitated. The heads were fixed in Bouin`s fixative and prepared routinely for paraffin sectioning and staining for histological and immunohistochemical investigations. Morphological and histological observations showed that 44.77% of cadmium treated pups affected with cleft palate, 13.51% of them with secondary palatal cleft. All control group animals were normally developed, as well as the pups of the remaining groups which treated with vitamin C and / or Nigella sativa with cadmium. Immunohistochemical examination of transforming growth factor alpha revealed that, control pups expressed a strong immune reaction in the primary palatal region in the palatine epithelium, while it was moderately positive in the underlying mesenchymal tissue cells. The reaction was strongly expressed in the endothelium of the blood capillaries and in the ossifying centers of the secondary palatal region. Cadmium treated mice (group II) revealed negative immune TGF-α reaction. Cadmium and vitamin C treated animals expressed moderate TGF-α reaction in the palatine epithelium and faint reaction in the underlying connective tissue cells, endothelium of the blood capillaries and in the ossifying centers. Animals treated with cadmium and Nigella sativa expressed the same immune reaction as in group III. Cadmium, vitamin C and Nigella sativa treated pups expressed strong immune reaction of TGF-α similar to that of control group.
[Shaymaa Hussein; Mohamed El-Sakhawy; Abdel-Aleem El-Saba; Abd Rabou M.I.; Hany Sherif and Abdel Razik Hashim. Histological and Immunohistochemical studies of the effect of vitamin C and Nigella sativa on the palate of albino mice’s offspring after cadmium exposure. Life Sci J 2013;10(1):2766-2772] (ISSN:1097-8135).http://www.lifesciencesite.com. 2
Keywords: Cadmium, palate, vitamin C, Nigella sativa and TGF-α